Babol-Pokora Katarzyna, Berent Jarosław
Department of Forensic Medicine, Medical University of Lodz, Łódź, Poland.
Acta Biochim Pol. 2008;55(4):815-9. Epub 2008 Nov 4.
SNP-minisequencing has become common in forensic genetics, especially for analysing degraded or low copy number DNA (LCN DNA). The aim of this study was to examine the usefulness of five SNP (single nucleotide polymorphism) markers for analyzing degraded and LCN DNA recovered from archival samples. DNA extractions of eight formalin-fixed paraffin-embedded (FFPE) tissues were performed and DNA fragments were amplified in one multiplex PCR (polymerase chain reaction). SNPs were identified in a minisequencing reaction and a gel electrophoresis in ABI Prism 377 Sequencer. The research confirmed the usefulness of SNP-minisequencing for analysing FFPE tissues.
单核苷酸多态性微测序技术在法医遗传学中已变得很常见，特别是用于分析降解的或低拷贝数DNA（LCN DNA）。本研究的目的是检验五个单核苷酸多态性（SNP）标记物对于分析从存档样本中回收的降解DNA和LCN DNA的有效性。对八个福尔马林固定石蜡包埋（FFPE）组织进行了DNA提取，并在一次多重聚合酶链反应（PCR）中扩增DNA片段。通过微测序反应和在ABI Prism 377测序仪中进行凝胶电泳来鉴定SNP。该研究证实了单核苷酸多态性微测序技术在分析FFPE组织方面的有效性。
