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影响向NtrB信号转导的PII T环突变也会消除酵母双杂交相互作用。

PII T-loop mutations affecting signal transduction to NtrB also abolish yeast two-hybrid interactions.

作者信息

Martínez-Argudo Isabel, Contreras Asunción

机构信息

División de Genética, Universidad de Alicante, E-03080 Alicante, Spain.

出版信息

J Bacteriol. 2002 Jul;184(13):3746-8. doi: 10.1128/JB.184.13.3746-3748.2002.

DOI:10.1128/JB.184.13.3746-3748.2002
PMID:12057972
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC135122/
Abstract

Mutations A49P and Delta47-53 at the T loop of the Escherichia coli GlnB (PII) protein impair regulatory interactions with the two-component sensor regulator NtrB (P. Jiang, P. Zucker, M. R. Atkinson, E. S. Kamberov, W. Tirasophon, P. Chandran, B. R. Schepke, and A. J. Ninfa, J. Bacteriol. 179: 4342-4353, 1997). We show here that these mutations also impair interactions between PII and NtrB in the yeast two-hybrid system, indicating that defects in NtrB regulation closely reflect binding impairment. The reported results underline the strength of two-hybrid assays for analysis of interactions involving the T loop of PII proteins.

摘要

大肠杆菌GlnB（PII）蛋白T环上的A49P和Delta47 - 53突变会损害与双组分传感器调节蛋白NtrB的调节相互作用（P. Jiang、P. Zucker、M. R. Atkinson、E. S. Kamberov、W. Tirasophon、P. Chandran、B. R. Schepke和A. J. Ninfa，《细菌学杂志》179: 4342 - 4353，1997年）。我们在此表明，这些突变在酵母双杂交系统中也会损害PII和NtrB之间的相互作用，这表明NtrB调节缺陷密切反映了结合受损。所报道的结果强调了双杂交分析在分析涉及PII蛋白T环的相互作用方面的优势。

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本文引用的文献

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J Bacteriol. 2002 Jan;184(1):200-6. doi: 10.1128/JB.184.1.200-206.2002.
2
Histidine kinases and response regulator proteins in two-component signaling systems.双组分信号系统中的组氨酸激酶和反应调节蛋白。
Trends Biochem Sci. 2001 Jun;26(6):369-76. doi: 10.1016/s0968-0004(01)01852-7.
3
Two-hybrid analysis of domain interactions involving NtrB and NtrC two-component regulators.涉及NtrB和NtrC双组分调节因子的结构域相互作用的双杂交分析。
Mol Microbiol. 2001 Apr;40(1):169-78. doi: 10.1046/j.1365-2958.2001.02369.x.
4
The structure of the PII-ATP complex.PII-ATP复合物的结构。
Eur J Biochem. 2001 Apr;268(7):2028-37. doi: 10.1046/j.1432-1327.2001.02074.x.
5
P(II) signal transduction proteins, pivotal players in microbial nitrogen control.P(II) 信号转导蛋白，微生物氮控制中的关键参与者。
Microbiol Mol Biol Rev. 2001 Mar;65(1):80-105. doi: 10.1128/MMBR.65.1.80-105.2001.
6
The Escherichia coli PII signal transduction protein regulates the activities of the two-component system transmitter protein NRII by direct interaction with the kinase domain of the transmitter module.大肠杆菌PII信号转导蛋白通过与信号转导模块的激酶结构域直接相互作用来调节双组分系统信号转导蛋白NRII的活性。
Biochemistry. 2000 Nov 7;39(44):13450-61. doi: 10.1021/bi000795m.
7
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J Bacteriol. 1999 Mar;181(6):1906-11. doi: 10.1128/JB.181.6.1906-1911.1999.
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J Mol Biol. 1998 Sep 11;282(1):149-65. doi: 10.1006/jmbi.1998.1979.
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