Mroczko Barbara, Szmitkowski Maciej, Okulczyk Bogna
Department of Biochemical Diagnostics, Medical Academy, Białystok, Poland.
Clin Chem Lab Med. 2002 Apr;40(4):351-5. doi: 10.1515/CCLM.2002.056.
We have investigated the serum level of granulocytecolony stimulating factor (G-CSF) and macrophagecolony stimulating factor (M-CSF) and the commonly accepted tumor markers, such as carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA 19-9) in colorectal cancer. Additionally, we have defined the diagnostic sensitivity, specificity, positive predictive value, negative predictive value and receiver-operating characteristics (ROC) curve for G-CSF and M-CSF. The serum levels of cytokines were measured in 49 patients with colorectal cancer and in 40 healthy subjects. G-CSF and M-CSF were determined using enzyme-linked immunosorbent assay (ELISA). CEA and CA 19-9 were measured by microparticle enzyme immunoassay. There were significant increases in the level of circulating G-CSF and M-CSF in the colorectal cancer patients compared to the control group. Moreover, the diagnostic sensitivity of M-CSF was higher (65%) than the sensitivity of CEA (31%) and CA 19-9 (20%). The diagnostic specificities of M-CSF and G-CSF were 95%, and the M-CSF predictive value was higher compared with the predictive value of G-CSF. These results suggest a potential role for M-CSF as a tumor marker for colorectal cancer.
我们研究了粒细胞集落刺激因子（G-CSF）和巨噬细胞集落刺激因子（M-CSF）的血清水平以及结直肠癌中常用的肿瘤标志物，如癌胚抗原（CEA）和糖类抗原19-9（CA 19-9）。此外，我们还确定了G-CSF和M-CSF的诊断敏感性、特异性、阳性预测值、阴性预测值以及受试者工作特征（ROC）曲线。对49例结直肠癌患者和40名健康受试者的细胞因子血清水平进行了检测。采用酶联免疫吸附测定（ELISA）法测定G-CSF和M-CSF。通过微粒酶免疫测定法检测CEA和CA 19-9。与对照组相比，结直肠癌患者循环G-CSF和M-CSF水平显著升高。此外，M-CSF的诊断敏感性（65%）高于CEA（31%）和CA 19-9（20%）的敏感性。M-CSF和G-CSF的诊断特异性均为95%，且M-CSF的预测值高于G-CSF的预测值。这些结果表明M-CSF作为结直肠癌肿瘤标志物具有潜在作用。
