Mroczko Barbara, Szmitkowski Maciej, Okulczyk Bogna
Department of Biochemical Diagnostics, Medical Academy, Białystok, Poland.
Clin Chem Lab Med. 2003 May;41(5):646-51. doi: 10.1515/CCLM.2003.098.
Hematopoietic growth factors (HGFs) are involved in the regulation of growth and spread of cancer. Therefore, in the present study, we have investigated in colorectal cancer patients the serum levels of selected HGFs, such as stem cell factor (SCF), interleukin 3 (IL-3), granulocyte-macrophage-colony stimulating factor (GM-CSF), and M-CSF in relation to controls and to the classical tumor markers, such as carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA 19-9) in colorectal cancer. Additionally, we have compared the serum levels of cytokines with tumor site and stage and other clinical characteristics such as age and sex. We also defined the receiver-operating characteristics (ROC) curve for HGFs and classical tumor markers. The tested cytokines were measured in 70 patients with colorectal cancer and in 40 healthy subjects. HGFs were determined using enzyme-linked immunosorbent assay (ELISA). CEA and CA 19-9 were measured by microparticle enzyme immunoassay. There were significant differences in the levels of circulating SCF, IL-3, M-CSF, GM-CSF, and CEA and CA 19-9 in the colorectal cancer patients compared to the control group. The levels of M-CSF and CEA were significantly higher in patients with a more advanced tumor stage. The significant positive correlation was observed between the CEA and CA 19-9 concentrations. The M-CSF serum levels correlated positively with the tested tumor markers. The M-CSF area under the ROC curve was the largest. These results suggest that M-CSF is, among the tested HGFs, the best candidate for a colorectal cancer tumor marker.
造血生长因子（HGFs）参与癌症的生长和扩散调节。因此，在本研究中，我们调查了结肠癌患者中所选HGFs的血清水平，如干细胞因子（SCF）、白细胞介素3（IL-3）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和M-CSF，并与对照组以及结肠癌中的经典肿瘤标志物，如癌胚抗原（CEA）和糖类抗原19-9（CA 19-9）进行比较。此外，我们还比较了细胞因子的血清水平与肿瘤部位、分期以及年龄和性别等其他临床特征。我们还定义了HGFs和经典肿瘤标志物的受试者操作特征（ROC）曲线。在70例结肠癌患者和40例健康受试者中检测了所测试的细胞因子。使用酶联免疫吸附测定（ELISA）法测定HGFs。通过微粒酶免疫测定法测量CEA和CA 19-9。与对照组相比，结肠癌患者循环中的SCF、IL-3、M-CSF、GM-CSF以及CEA和CA 19-9水平存在显著差异。肿瘤分期较晚的患者中M-CSF和CEA水平显著更高。CEA和CA 19-9浓度之间存在显著正相关。M-CSF血清水平与所测试的肿瘤标志物呈正相关。ROC曲线下M-CSF的面积最大。这些结果表明，在所测试的HGFs中，M-CSF是结肠癌肿瘤标志物的最佳候选者。
