Siret René, Gigaud Olivier, Rosec Jean Philippe, This Patrice
Unité Mixte de Recherches 1097, Diversité et Génomes des Plantes Cultivées (ENSA.M, CIRAD, INRA, IRD), Equipe Génétique Vigne, ENSA.M-INRA, 2 Place P. Viala, 34060 Montpellier, France.
J Agric Food Chem. 2002 Jun 19;50(13):3822-7. doi: 10.1021/jf011462e.
Because wine quality highly relies on the varietal composition of the must, the development of methods allowing the authentication of varieties in musts and wines would be of great value as a guarantee of quality. Microsatellite markers have already been applied to the authentication of grape juices (Faria, M. A.; Magalhães, R.; Ferreira, M. A.; Meredith, C. P.; Ferreira Monteiro, F. J. Agric. Food Chem. 2000, 48, 1096-1100) and to the analysis of experimental wines (Siret, R.; Boursiquot, J. M.; Merle, M. H.; Cabanis, J. C.; This, P. J. Agric Food Chem. 2000, 48, 5035-5040). In the present paper, we accessed the usefulness of this technology for the analysis of must and wine mixtures. The detection limit of DNA mixtures was first estimated on DNA extracted from leaves: 4% of a foreign DNA can be detected. Analysis of must and wine mixtures (Chardonnay B/Clairette B and Syrah N/Grenache N) was performed on experimental fermentations. DNA was extracted along the fermentation process and analyzed using five microsatellite loci. The 70:30 (v/v) mixtures were successfully analyzed until the end of the fermentation. The applications of these results to commercial purposes are discussed.
由于葡萄酒的品质高度依赖于葡萄汁的品种组成，因此开发能够鉴定葡萄汁和葡萄酒中品种的方法对于保证品质具有重要价值。微卫星标记已被应用于葡萄汁的鉴定（法里亚，M.A.；马加良斯，R.；费雷拉，M.A.；梅雷迪思，C.P.；费雷拉·蒙泰罗，F.《农业与食品化学杂志》2000年，48卷，1096 - 1100页）以及实验葡萄酒的分析（西雷，R.；布尔西夸，J.M.；梅尔，M.H.；卡巴尼斯，J.C.；蒂斯，P.《农业与食品化学杂志》2000年，48卷，5035 - 5040页）。在本文中，我们评估了这项技术用于分析葡萄汁和葡萄酒混合物的实用性。首先在从叶片提取的DNA上估计DNA混合物的检测限：可以检测到4%的外源DNA。对实验发酵过程中的葡萄汁和葡萄酒混合物（霞多丽B/克莱雷特B以及西拉N/歌海娜N）进行了分析。在发酵过程中提取DNA，并使用五个微卫星位点进行分析。70:30（v/v）的混合物在发酵结束前都成功得到了分析。讨论了这些结果在商业用途上的应用。
