Faria M A, Magalhães R, Ferreira M A, Meredith C P, Monteiro F F
CEQUP/Serviço de Bromatologia, Faculdade de Farmácia, Universidade do Porto, Rua de Aníbal Cunha, 4050-047 Porto, Portugal.
J Agric Food Chem. 2000 Apr;48(4):1096-100. doi: 10.1021/jf990837h.
A microsatellite DNA-based method for Vitis vinifera grape must authentication is presented. Five of the most important port wine producing grape cultivars (Tinta Roriz, Tinto Cão, Touriga Francesa, Touriga Nacional, and Tinta Barroca) were typed at four microsatellite loci described by Bowers et al. (Genome 1996, 39, 628-633) and Thomas and Scott (Theor. Appl. Genet. 1993, 86, 985-990). The corresponding 5 varietal musts and 26 must mixtures that result from the combination of the five varieties were also typed at the four loci. There were no differences between the corresponding leaf and varietal must profiles. All must combinations showed the expected band profiles corresponding to the sum of the varietal band profile components. Among the 26 must mixtures, 8 could be discriminated using the four loci.
本文介绍了一种基于微卫星DNA的用于鉴定酿酒葡萄葡萄汁的方法。对五个最重要的波特酒酿造葡萄品种（罗丽红、国产多瑞加、法国多瑞加、多瑞加国民、巴罗卡）在Bowers等人（《基因组》，1996年，第39卷，628 - 633页）以及Thomas和Scott（《理论与应用遗传学》，1993年86卷，985 - 990页）描述的四个微卫星位点进行分型。相应的5种单一品种葡萄汁以及由这五个品种组合而成的26种混合葡萄汁也在这四个位点进行分型。相应的叶片和单一品种葡萄汁图谱之间没有差异。所有的葡萄汁组合都显示出与单一品种条带图谱成分总和相对应的预期条带图谱。在这26种混合葡萄汁中，有8种可以通过这四个位点进行区分。
