从真菌和链霉菌中分离抑制多巴脱羧酶的异黄酮。

Isolation of isoflavones inhibiting DOPA decarboxylase from fungi and streptomyces.

作者信息

Umezawa H, Tobe H, Shibamoto N, Nakamura F, Nakamura K

出版信息

J Antibiot (Tokyo). 1975 Dec;28(12):947-52. doi: 10.7164/antibiotics.28.947.

DOI:10.7164/antibiotics.28.947

PMID:1206008

Abstract

By screening of culture filtrates of fungi and streptomyces for activity in inhibit dopa decarboxylase the following isoflavone compounds were obtained: psi-tectorigenen (I), genistein (II), orobol (IV), 8-hydroxygenistein (V) and a new compound (III). III was elucidated to be 3', 4', 5, 7-tetrahydroxy-8methoxy isoflavone. Among these isoflavones, IV and III showed the strongest activity in inhibiting dopa decarboxylase. All these isoflavones also inhibited histidine decarboxylase and catechol-O-methyltrasnferase. Activities of these compounds to inhibit tyrosine hydroxylase and dopamine beta-hydroxylase were examined. Orobol which showed no or only slight inhibition of tyrosine hydroxylase and dopamine beta-hydroxylase exhibited a significant hypotensive effect on spontaneously hypertensive rats.

摘要

通过筛选真菌和链霉菌的培养滤液中抑制多巴脱羧酶的活性，获得了以下异黄酮化合物：次大豆黄素（I）、染料木黄酮（II）、芒柄花黄素（IV）、8-羟基染料木黄酮（V）和一种新化合物（III）。经鉴定，III为3'，4'，5，7-四羟基-8-甲氧基异黄酮。在这些异黄酮中，IV和III在抑制多巴脱羧酶方面表现出最强的活性。所有这些异黄酮还抑制组氨酸脱羧酶和儿茶酚-O-甲基转移酶。检测了这些化合物抑制酪氨酸羟化酶和多巴胺β-羟化酶的活性。对酪氨酸羟化酶和多巴胺β-羟化酶无抑制作用或仅有轻微抑制作用的芒柄花黄素对自发性高血压大鼠表现出显著的降压作用。