Seefeld M D, Albrecht R M, Peterson R E
Toxicology. 1979 Nov;14(3):263-72. doi: 10.1016/0300-483x(79)90008-8.
To assess effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on liver function adult male rhesus monkeys were treated with a single oral dose of acetone/corn oil (control) or 5, 25, or 75 micrograms/kg TCDD. Each monkey was used as its own control and indocyanine green (ICG) blood clearance and the following serum enzymes: glutamic pyruvate transaminase (SGPT), sorbitol dehydrogenase (SDH) and gamma glutamyl transpeptidase (gamma GTP), were measured at regular intervals for 4 weeks before and 17 weeks after treatment. In control monkeys ICG blood clearance and serum enzymes were similar before and after treatment. However, in the monkey that received 5 micrograms/kg TCDD there was a mild increase in ICG blood clearance followed by a slight decrease. The magnitude of this biphasic change was greater in monkeys that received 25 and 75 micrograms/kg TCDD and the decrease in clearance was invariably associated with a 1--2-week period before the monkeys died. SDH and SGPT activities were elevated at some time during the course of intoxication in all TCDD-treated monkeys but gamma GTP activity was not altered. The monkey treated with 5 micrograms/kg TCDD survived but monkeys treated with 25 and 75 micrograms/kg died 4--6 weeks after treatment. Light microscopy of the livers of TCDD-treated monkeys that died revealed fatty infiltration with minimal hepatocellular necrosis.
为评估2,3,7,8-四氯二苯并-对-二恶英（TCDD）对成年雄性恒河猴肝功能的影响，给成年雄性恒河猴单次口服丙酮/玉米油（对照组）或5、25或75微克/千克TCDD。每只猴子自身作为对照，在治疗前4周和治疗后17周定期测量吲哚菁绿（ICG）血液清除率以及以下血清酶：谷丙转氨酶（SGPT）、山梨醇脱氢酶（SDH）和γ-谷氨酰转肽酶（γGTP）。在对照猴子中，治疗前后ICG血液清除率和血清酶相似。然而，在接受5微克/千克TCDD的猴子中，ICG血液清除率轻度升高，随后略有下降。在接受25和75微克/千克TCDD的猴子中，这种双相变化的幅度更大，清除率的下降总是与猴子死亡前1至2周的时间段相关。在所有接受TCDD治疗的猴子中毒过程中的某些时候，SDH和SGPT活性升高，但γGTP活性未改变。接受5微克/千克TCDD治疗的猴子存活下来，但接受25和75微克/千克TCDD治疗的猴子在治疗后4至6周死亡。对死亡的接受TCDD治疗的猴子的肝脏进行光学显微镜检查发现有脂肪浸润，肝细胞坏死极少。
