Seefeld M D, Albrecht R M, Gilchrist K W, Peterson R E
Arch Environ Contam Toxicol. 1980;9(3):317-27. doi: 10.1007/BF01057411.
The objective was to determine if a blood clearance test would detect liver dysfunction in rats and rabbits treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Guinea pigs were included as a negative control, because TCDD does not produce detectable hepatotoxicity in this species. TCDD was given as a single dose to male rats (25 microgram/kg, po), rabbits (25 microgram/kg, ip) and guinea pigs (2 microgram/kg, ip) and liver function was assessed 10 days later by determining blood clearance of indocyanine green (ICG) and ouabain. Activity in serum of sorbitol dehydrogenase (SDH), glutamic pyruvic transaminase (SGPT) and gamma glutamyl transpeptidase (gamma GTP) were also measured and light microscopy performed on the liver. The results showed that hepatotoxicity in the rabbit could be detected by a reduction in ICG blood clearance and an elevation in SDH activity. In the rat, ouabain blood clearance was reduced and SDH activity elevated. None of the liver function tests were altered in the guinea pig. These results underline the usefulness of blood clearance tests using ICG and ouabain in detecting TCDD hepatotoxicity in animals.
目的是确定血液清除试验能否检测出用2,3,7,8-四氯二苯并-对-二恶英（TCDD）处理的大鼠和兔子的肝功能障碍。将豚鼠作为阴性对照，因为TCDD在该物种中不会产生可检测到的肝毒性。给雄性大鼠（25微克/千克，口服）、兔子（25微克/千克，腹腔注射）和豚鼠（2微克/千克，腹腔注射）单次给予TCDD，并在10天后通过测定吲哚菁绿（ICG）和哇巴因的血液清除率来评估肝功能。还测量了血清中山梨醇脱氢酶（SDH）、谷丙转氨酶（SGPT）和γ-谷氨酰转肽酶（γGTP）的活性，并对肝脏进行了光学显微镜检查。结果表明，可通过ICG血液清除率降低和SDH活性升高来检测兔子的肝毒性。在大鼠中，哇巴因血液清除率降低，SDH活性升高。豚鼠的肝功能测试均未改变。这些结果强调了使用ICG和哇巴因的血液清除试验在检测动物TCDD肝毒性方面的有用性。
