da Silva Edson R, Castilho Tiago M, Pioker Fabiana C, Tomich de Paula Silva Carlos H, Floeter-Winter Lucile M
Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374 São Paulo, SP 05508-900, Brazil.
Int J Parasitol. 2002 Jun;32(6):727-37. doi: 10.1016/s0020-7519(02)00002-4.
The genomic organisation of the gene encoding Leishmania (Leishmania) amazonensis arginase as well as its flanking regions were characterised. The size of the transcribed RNA was determined, allowing us to map the genomic sites signalling for RNA trans-splicing and putative polyadenylation regions. The general organisation was compared with genes encoding other proteins already described in organisms of the Trypanosomatid family. The complete nucleotide sequence of the arginase open reading frame was obtained and the three-dimensional structure of the enzyme was inferred by a computational analysis of the deduced amino acid sequence, based on the established crystal structure described for Rattus norvergicus arginase. The human liver arginase sequence was analysed in the same way and the comparison of the presumed structure of both the Leishmania and human enzymes identified some differences that may be exploited in chemotherapeutic studies.
对编码亚马逊利什曼原虫（利什曼原虫属）精氨酸酶的基因及其侧翼区域的基因组组织进行了表征。确定了转录RNA的大小，这使我们能够绘制出指示RNA反式剪接的基因组位点和假定的聚腺苷酸化区域。将其总体组织与锥虫科生物中已描述的其他蛋白质编码基因进行了比较。获得了精氨酸酶开放阅读框的完整核苷酸序列，并基于已确定的褐家鼠精氨酸酶晶体结构，通过对推导的氨基酸序列进行计算分析，推断出该酶的三维结构。以同样的方式分析了人肝精氨酸酶序列，利什曼原虫和人酶假定结构的比较确定了一些差异，这些差异可能在化疗研究中得到利用。
