Campos Y R, Carvalho O S, Goveia C O, Romanha A J
Centro de Pesquisas René Rachou-FIOCRUZ, Avenida Augusto de Lima 1715, Barro Preto, CEP 30190-002, MG, Belo Horizonte, Brazil.
Acta Trop. 2002 Jul;83(1):19-27. doi: 10.1016/s0001-706x(02)00051-7.
The genetic variability of Brazilian Biomphalaria glabrata populations was studied using SSR-PCR. This technique is a variant of the polymerase chain reaction (PCR), which consists of using a single primer directed towards microsatellite regions under high stringency reaction conditions. Twenty snails of each population from eight distant Brazilian localities were analyzed. Morphology and PCR-RFLP were used for previous specific identification of the snails. Bands generated after gel electrophoresis of the SSR-PCR products of each snail were used to study intra- and interpopulation genetic variability. Fifty-five prominent bands were considered in a pairwise band comparison for the determination of genetic variability. Genetic variability was greater between populations than within populations. Snail populations from the field and the laboratory presented almost no genetic differences. No relationship between genetic variability and geographic distance was found. SSR-PCR proved to be a good alternative molecular tool for the population study of B. glabrata.
利用SSR-PCR技术研究了巴西光滑双脐螺种群的遗传变异性。该技术是聚合酶链反应（PCR）的一种变体，它包括在高严格反应条件下使用一个针对微卫星区域的单一引物。对来自巴西八个偏远地区的每个种群的20只蜗牛进行了分析。形态学和PCR-RFLP用于之前对蜗牛的特异性鉴定。每只蜗牛的SSR-PCR产物经凝胶电泳后产生的条带用于研究种群内和种群间的遗传变异性。在成对条带比较中考虑了55条突出条带以确定遗传变异性。种群间的遗传变异性大于种群内的遗传变异性。来自野外和实验室的蜗牛种群几乎没有遗传差异。未发现遗传变异性与地理距离之间的关系。SSR-PCR被证明是研究光滑双脐螺种群的一种很好的替代分子工具。
