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[多杀性巴氏杆菌脱羧酶活性研究]

[Decarboxylase activity study of Pasteurella multocida].

作者信息

Karaivanov L

出版信息

Vet Med Nauki. 1979;16(7):49-53.

PMID:120631
Abstract

Ornithine-, lysine- and arginine-decarboxylase activity of 218 P. multocida strains, isolated from birds of varying disease symptoms in Bulgaria and CSSR, and from pigs, rabbits and birds in Cuba, USSR and CSSR, was studied after the method of Moller. Positive ornithine decarboxylase activity was established in 211 strains, low -- in 2, and negative -- in 5 strains. Low arginine decarboxylase activity was observed in 12 Pasteurella strains, while in 14 -- low lysine decarboxylase activity. The presence of ornithine decarboxylase activity can be used, along with the cultural and biochemical properties and with lyzation by a specific bacteriophage, as a taxonomic character for the species. All Pasteurella strains pathogenic for white mice, produce ornithine-decarboxylase. Lines of the strain X 73 obtained following gamma-irradiation having lost their ornithine-decarboxylase are pathogenic for white mice.

摘要

采用莫勒方法研究了从保加利亚和捷克斯洛伐克有不同疾病症状的禽类以及古巴、苏联和捷克斯洛伐克的猪、兔和禽类中分离出的218株多杀性巴氏杆菌的鸟氨酸、赖氨酸和精氨酸脱羧酶活性。在211株中检测到鸟氨酸脱羧酶活性呈阳性,2株呈低活性,5株呈阴性。在12株巴氏杆菌中观察到精氨酸脱羧酶活性较低,14株中赖氨酸脱羧酶活性较低。鸟氨酸脱羧酶活性的存在可与培养和生化特性以及特定噬菌体裂解一起用作该物种的分类特征。所有对白鼠致病的巴氏杆菌菌株都产生鸟氨酸脱羧酶。经γ射线照射后获得的X 73菌株系失去了鸟氨酸脱羧酶活性,但对白鼠仍具有致病性。

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