Bentley David
Department of Biochemistry and Molecular Genetics, UCHSC, B121, 4200 East 9th Avenue, Denver, CO 80262, USA.
Curr Opin Cell Biol. 2002 Jun;14(3):336-42. doi: 10.1016/s0955-0674(02)00333-2.
Processing of RNA precursors to their mature form often occurs co-transcriptionally. Consequently, the ternary complex of DNA template, RNA polymerase and nascent RNA chain is the physiological substrate for factors that modify the nascent RNA by capping, splicing and cleavage/polyadenylation. mRNA production is thought to occur within a "factory" that contains the RNA polymerase II transcription machine and the processing machines. Newly discovered protein-protein contacts between RNA polymerase and factors that process mRNA precursors are beginning to illuminate how the "mRNA factory" works.
RNA前体加工成成熟形式的过程通常在转录过程中同时发生。因此，DNA模板、RNA聚合酶和新生RNA链的三元复合物是通过加帽、剪接和切割/聚腺苷酸化修饰新生RNA的因子的生理底物。mRNA的产生被认为发生在一个包含RNA聚合酶II转录机器和加工机器的“工厂”内。RNA聚合酶与加工mRNA前体的因子之间新发现的蛋白质-蛋白质相互作用开始揭示“mRNA工厂”的工作方式。
