Estuningsih S, Soedarmanto I, Fink K, Lämmler C, Wibawan I W T
Institut für Tierärztliche Nahrungsmittelkunde, Justuts-Liebig-Universitat Giessen, Germany.
J Vet Med B Infect Dis Vet Public Health. 2002 May;49(4):185-7. doi: 10.1046/j.1439-0450.2002.00482.x.
All 83 bacterial strains isolated from seven farms in three areas of the island of Java in Indonesia investigated in the present study could be identified as Streptococcus agalactiae. Identification was performed by cultural, biochemical and serological properties and by polymerase chain reaction amplification of species-specific parts of the gene encoding the 16S rRNA, the 16S-23S rDNA intergenic spacer region and the CAMP factor (cfb) gene. All isolates were unpigmented. almost all of the isolates had the serotype pattern II/X. Despite these similarities a macrorestriction analysis of the chromosomal DNA of the bacteria revealed no significant homologies of the DNA-fingerprints of the S. agalactiae from the various areas. This last finding might possibly indicate that a single ancestral unpigmented serotype II/X S. agalactiae clone was responsible for the mastitis situation on Java and had evolved separately in the various farms and regions.
在本研究中，对从印度尼西亚爪哇岛三个地区的七个农场分离出的83株细菌菌株进行调查，所有菌株均可鉴定为无乳链球菌。通过培养、生化和血清学特性以及对编码16S rRNA、16S - 23S rDNA基因间隔区和CAMP因子（cfb）基因的物种特异性部分进行聚合酶链反应扩增来进行鉴定。所有分离株均无色素沉着。几乎所有分离株都具有II/X血清型模式。尽管存在这些相似性，但对细菌染色体DNA的宏观限制性分析表明，来自不同地区的无乳链球菌的DNA指纹没有明显的同源性。这一最终发现可能表明，一个单一的无色素沉着祖先II/X血清型无乳链球菌克隆导致了爪哇岛的乳腺炎情况，并且在各个农场和地区分别进化。
