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本文引用的文献

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Determination of intraspecies variations of the V2 region of the 16S rRNA gene of Streptococcus equi subsp. zooepidemicus.马链球菌兽疫亚种16S rRNA基因V2区域种内变异的测定。
Res Vet Sci. 2000 Feb;68(1):33-9. doi: 10.1053/rvsc.1999.0332.
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Amplification of 16S ribosomal RNA gene sequences for the identification of streptococci of Lancefield group B.扩增16S核糖体RNA基因序列以鉴定B群兰氏链球菌。
Res Vet Sci. 1999 Oct;67(2):159-62. doi: 10.1053/rvsc.1998.0298.
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Properties of serological group B streptococci of dog, cat and monkey origin.犬、猫和猴源血清学B群链球菌的特性
Zentralbl Veterinarmed B. 1998 Nov;45(9):561-6. doi: 10.1111/j.1439-0450.1998.tb00827.x.
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Species identification of Streptococcus porcinus by restriction fragment length polymorphism analysis of 16S ribosomal DNA.通过16S核糖体DNA的限制性片段长度多态性分析对猪链球菌进行菌种鉴定。
Res Vet Sci. 1998 Jul-Aug;65(1):85-6. doi: 10.1016/s0034-5288(98)90033-9.
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Phylogenetic diversity of Streptococcus suis strains of various serotypes as revealed by 16S rRNA gene sequence comparison.通过16S rRNA基因序列比较揭示的不同血清型猪链球菌菌株的系统发育多样性。
Int J Syst Bacteriol. 1998 Apr;48 Pt 2:581-9. doi: 10.1099/00207713-48-2-581.
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Identification of staphylococcal and streptococcal causes of bovine mastitis using 16S-23S rRNA spacer regions.利用16S - 23S rRNA间隔区鉴定引起牛乳腺炎的葡萄球菌和链球菌
Microbiology (Reading). 1997 Nov;143 ( Pt 11):3491-3500. doi: 10.1099/00221287-143-11-3491.
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Characterization of the Lancefield group C streptococcus 16S-23S RNA gene intergenic spacer and its potential for identification and sub-specific typing.兰斯菲尔德C群链球菌16S - 23S RNA基因间隔区的特征分析及其在鉴定和亚特异性分型中的潜力。
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Development and use of species-specific oligonucleotide probes for differentiation of Streptococcus uberis and Streptococcus parauberis.用于区分乳房链球菌和副乳房链球菌的种特异性寡核苷酸探针的开发与应用。
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Development of gene probes for the specific identification of Streptococcus uberis and Streptococcus parauberis based upon large subunit rRNA gene sequences.基于大亚基rRNA基因序列开发用于特异性鉴定乳房链球菌和副乳房链球菌的基因探针。
J Appl Bacteriol. 1993 May;74(5):526-31.
10
Development of PCR-based hybridization protocol for identification of streptococcal species.用于鉴定链球菌种类的基于聚合酶链反应的杂交方案的开发。
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用于快速鉴定和区分乳房链球菌和副乳房链球菌的聚合酶链反应方法的评估

Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis.

作者信息

Hassan A A, Khan I U, Abdulmawjood A, Lämmler C

机构信息

Institut für Tierärztliche Nahrungsmittelkunde, Bakteriologie und Hygiene der Milch, der Justus-Liebig-Universität Giessen, Giessen, Germany.

出版信息

J Clin Microbiol. 2001 Apr;39(4):1618-21. doi: 10.1128/JCM.39.4.1618-1621.2001.

DOI:10.1128/JCM.39.4.1618-1621.2001
PMID:11283100
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC87983/
Abstract

Streptococcus uberis and Streptococcus parauberis reference strains and isolates obtained from routine diagnostics were investigated by PCR with oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene, the 23S rRNA gene, and the 16S-23S rRNA intergenic spacer region of both species. All three primer pairs allowed an identification of 67 isolates as S. uberis and 4 isolates as S. parauberis.

摘要

使用根据乳房链球菌（Streptococcus uberis）和副乳房链球菌（Streptococcus parauberis）的16S rRNA基因、23S rRNA基因以及这两个物种的16S - 23S rRNA基因间隔区的物种特异性部分设计的寡核苷酸引物，通过聚合酶链反应（PCR）对乳房链球菌和副乳房链球菌的参考菌株以及从常规诊断中获得的分离株进行了研究。所有三对引物都能将67株分离株鉴定为乳房链球菌，4株分离株鉴定为副乳房链球菌。