用于快速鉴定和区分乳房链球菌和副乳房链球菌的聚合酶链反应方法的评估
Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis.
作者信息
Hassan A A, Khan I U, Abdulmawjood A, Lämmler C
机构信息
Institut für Tierärztliche Nahrungsmittelkunde, Bakteriologie und Hygiene der Milch, der Justus-Liebig-Universität Giessen, Giessen, Germany.
出版信息
J Clin Microbiol. 2001 Apr;39(4):1618-21. doi: 10.1128/JCM.39.4.1618-1621.2001.
Abstract
Streptococcus uberis and Streptococcus parauberis reference strains and isolates obtained from routine diagnostics were investigated by PCR with oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene, the 23S rRNA gene, and the 16S-23S rRNA intergenic spacer region of both species. All three primer pairs allowed an identification of 67 isolates as S. uberis and 4 isolates as S. parauberis.
摘要
使用根据乳房链球菌（Streptococcus uberis）和副乳房链球菌（Streptococcus parauberis）的16S rRNA基因、23S rRNA基因以及这两个物种的16S - 23S rRNA基因间隔区的物种特异性部分设计的寡核苷酸引物，通过聚合酶链反应（PCR）对乳房链球菌和副乳房链球菌的参考菌株以及从常规诊断中获得的分离株进行了研究。所有三对引物都能将67株分离株鉴定为乳房链球菌，4株分离株鉴定为副乳房链球菌。
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