Abiraman S, Varma H K, Umashankar P R, John Annie
Implant Biology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.
Biomaterials. 2002 Jul;23(14):3023-31. doi: 10.1016/s0142-9612(02)00064-9.
Fibrin sealant or fibrin glue (FG) has been found to be effective as a wound-healing substance in surgery. However, its role in bone fracture healing and osseous tissue response is not fully understood. This ambiguity questions the potential of FG as an inductive protein. The present study was undertaken to evaluate the osteoinductive property of FG when coated with calcium phosphate and glass ceramics and implanted in the extraskeletal site of male Swiss albino mice. Implant materials used for this study were hydroxyapatite (HA) porous granules (300-350 microm), bioactive glass system (BGS)-AW type and calcium phosphate calcium silicate system (HABGS) non-porous granules (300-350 microm). Uncoated granules (control) and coated granules with 2.5 mg FG and 5 mg FG were implanted in the quadriceps muscle of mice and sacrificed after 28 days. Histologically, HA, BGS and HABGS implanted animal groups showed good healing response. However, neo-osteogenesis was observed only in the BGS and HABGS granules impregnated with FG. Furthermore, bone formation was observed to be more conspicuous in 5 mg FG coated BGS and HABGS granules when compared with 2.5 mg FG coated BGS and HABGS granules. Fluorochrome labeling proved that mineralization had already started by day 15 with FG preadsorbed BGS and HABGS granules. On the contrary, the uncoated granules did not show any de novo bone formation. This experimental study provides an evidence of the positive role of FG as a potential osteoinductive biologic tissue adhesive.
纤维蛋白密封剂或纤维蛋白胶（FG）已被发现在手术中作为一种伤口愈合物质是有效的。然而，其在骨折愈合和骨组织反应中的作用尚未完全明确。这种不确定性对FG作为一种诱导蛋白的潜力提出了质疑。本研究旨在评估涂覆有磷酸钙和玻璃陶瓷并植入雄性瑞士白化小鼠骨骼外部位的FG的骨诱导特性。本研究使用的植入材料为羟基磷灰石（HA）多孔颗粒（300 - 350微米）、生物活性玻璃系统（BGS）- AW型以及磷酸钙硅酸钙系统（HABGS）无孔颗粒（300 - 350微米）。将未涂覆的颗粒（对照）以及涂覆有2.5毫克FG和5毫克FG的颗粒植入小鼠股四头肌，并在28天后处死。组织学上，植入HA、BGS和HABGS的动物组显示出良好的愈合反应。然而，仅在浸渍有FG的BGS和HABGS颗粒中观察到了新骨形成。此外，与涂覆2.5毫克FG的BGS和HABGS颗粒相比，在涂覆5毫克FG的BGS和HABGS颗粒中观察到骨形成更为明显。荧光染料标记证明，在第15天时，预吸附有FG的BGS和HABGS颗粒已经开始矿化。相反，未涂覆的颗粒未显示任何新生骨形成。这项实验研究为FG作为一种潜在的骨诱导生物组织粘合剂的积极作用提供了证据。
