Le Nihouannen Damien, Saffarzadeh Afchine, Aguado Eric, Goyenvalle Eric, Gauthier Olivier, Moreau Françoise, Pilet Paul, Spaethe Reiner, Daculsi Guy, Layrolle Pierre
Inserm, U791, Nantes, France.
J Mater Sci Mater Med. 2007 Feb;18(2):225-35. doi: 10.1007/s10856-006-0684-7.
Calcium phosphate (Ca-P) ceramics are currently used in various types of orthopaedic and maxillofacial applications because of their osteoconductive properties. Fibrin glue is also used in surgery due to its haemostatic, chemotactic and mitogenic properties and also as scaffolds for cell culture and transplantation. In order to adapt to surgical sites, bioceramics are shaped in blocks or granules and preferably in porous forms. Combining these bioceramics with fibrin glue provides a mouldable and self-hardening composite biomaterial. The aim of this work is to study the osteogenic properties of this composite material using two different animal models. The formation of newly formed bone (osteoinduction) and bone healing capacity (osteconduction) have been study in the paravertebral muscles of sheep and in critical sized defects in the femoral condyle of rabbits, respectively. The different implantations sites were filled with composite material associating Ca-P granules and fibrin glue. Ca-P granules of 1-2 mm were composed with 60% of hydroxyapatite and 40% of beta tricalcium phosphate in weight. The fibrin glue was composed of fibrinogen, thrombin and other biological factors. After both intramuscular or intraosseous implantations for 24 weeks and 3, 6, 12 and 24 weeks, samples were analyzed using histology and histomorphometry and mechanical test. In all cases, the newly formed bone was observed in close contact and around the ceramic granules. Depending on method of quantification, 6.7% (with BSEM) or 17% (with micro CT) of bone had formed in the sheep muscles and around 40% in the critical sized bone rabbit defect after 24 weeks. The Ca-P/fibrin material could be used for filling bone cavities in various clinical indications.
磷酸钙（Ca-P）陶瓷由于其骨传导特性，目前被用于各种骨科和颌面应用中。纤维蛋白胶因其止血、趋化和促有丝分裂特性，也被用于外科手术，还可作为细胞培养和移植的支架。为了适应手术部位，生物陶瓷被制成块状或颗粒状，最好是多孔形式。将这些生物陶瓷与纤维蛋白胶结合，可提供一种可塑形且能自硬化的复合生物材料。这项工作的目的是使用两种不同的动物模型研究这种复合材料的成骨特性。分别在绵羊的椎旁肌和兔股骨髁的临界尺寸缺损处研究了新形成骨的形成（骨诱导）和骨愈合能力（骨传导）。不同的植入部位填充了含有Ca-P颗粒和纤维蛋白胶的复合材料。1-2毫米的Ca-P颗粒按重量计由60%的羟基磷灰石和40%的β-磷酸三钙组成。纤维蛋白胶由纤维蛋白原、凝血酶和其他生物因子组成。在肌肉内或骨内植入24周以及3、6、12和24周后，使用组织学、组织形态计量学和力学测试对样本进行分析。在所有情况下，都观察到新形成的骨与陶瓷颗粒紧密接触并围绕着陶瓷颗粒。根据量化方法，24周后，绵羊肌肉中形成了6.7%（使用背散射电子显微镜）或17%（使用微型计算机断层扫描）的骨，在兔临界尺寸骨缺损处形成了约40%的骨。Ca-P/纤维蛋白材料可用于填充各种临床适应症中的骨腔。
