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钠离子促进骨组织再生的研究：大鼠颅骨的实验研究

Sodium-DNA for Bone Tissue Regeneration: An Experimental Study in Rat Calvaria.

机构信息

Section of Anatomy and Physiopathology, Department of Clinical and Experimental Sciences, University of Brescia, Brescia, Italy.

Interdipartimental University Center of Research "Adaption and Regeneration of Tissues and Organs (ARTO)", University of Brescia, Brescia, Italy.

出版信息

Biomed Res Int. 2017;2017:7320953. doi: 10.1155/2017/7320953. Epub 2017 Sep 10.

DOI:10.1155/2017/7320953
PMID:29082254
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5610838/
Abstract

Surgical techniques in dental and maxillofacial surgery request fast bone tissue regeneration, so there is a significant need to improve therapy for bone regeneration. Several studies have recently underlined the importance of nucleotides and nucleosides to increase cell proliferation and activity; in particular, the ability of polydeoxyribonucleotide (PDRN) to induce growth and activity of human osteoblasts was demonstrated. Sodium-DNA is the deoxyribonucleic acid (DNA) extracted from the gonadic tissue of male sturgeon and then purified, depolymerized, and neutralized with sodium hydroxide. To date, there are no evidences about the use of Sodium-DNA for bone tissue regeneration. Consequently, our question is about the efficacy of Sodium-DNA in bone healing. For testing the role of Sodium-DNA in bone healing we used a rat calvarial defect model. Sodium-DNA at different concentrations used alone or in association with Fibrin and/or Bio-Oss was used for healing treatments and the bone healing process was evaluated by histomorphometric and immunohistochemical analyses. Our results suggested a positive effect of Sodium-DNA in bone regeneration, providing a useful protocol and a model for the future clinical evaluation of its osteogenic properties.

摘要

口腔颌面外科手术技术要求快速的骨组织再生，因此需要显著改进骨再生的治疗方法。最近的几项研究强调了核苷酸和核苷对于增加细胞增殖和活性的重要性；特别是多聚脱氧核苷酸（PDRN）能够诱导人成骨细胞的生长和活性。钠-DNA 是从雄性鲟鱼性腺组织中提取并经过纯化、解聚和氢氧化钠中和得到的脱氧核糖核酸（DNA）。迄今为止，尚无关于钠-DNA 用于骨组织再生的证据。因此，我们的问题是关于钠-DNA 在骨愈合中的功效。为了测试钠-DNA 在骨愈合中的作用，我们使用了大鼠颅骨缺损模型。单独使用或与纤维蛋白和/或 Bio-Oss 联合使用不同浓度的钠-DNA 进行愈合治疗，并通过组织形态计量学和免疫组织化学分析评估骨愈合过程。我们的结果表明，钠-DNA 对骨再生具有积极作用，为其成骨特性的未来临床评估提供了有用的方案和模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9225/5610838/6beafde0d0fe/BMRI2017-7320953.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9225/5610838/c209fc59318a/BMRI2017-7320953.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9225/5610838/ae7d0ac7ae1e/BMRI2017-7320953.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9225/5610838/245c2cac8108/BMRI2017-7320953.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9225/5610838/6beafde0d0fe/BMRI2017-7320953.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9225/5610838/c209fc59318a/BMRI2017-7320953.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9225/5610838/ae7d0ac7ae1e/BMRI2017-7320953.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9225/5610838/245c2cac8108/BMRI2017-7320953.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9225/5610838/6beafde0d0fe/BMRI2017-7320953.004.jpg

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