Jones G E, Royle L G, Murray L
Appl Environ Microbiol. 1979 Nov;38(5):800-5. doi: 10.1128/aem.38.5.800-805.1979.
Twenty-two species of bacteria of marine, estuarine, and terrestrial origin were analyzed for cationic content by atomic absorption spectrophotometry after growth in a basal seawater medium. Alcaligenes marinus was analyzed from eight separate but replicate determinations yielding the following cationic concentrations: Na, 5,600 +/- 2,260; Mg 1,580 +/- 740; K, 700 +/- 360; Ca, 790 +/- 390; Mn, 1.7 +/- 0.5; Fe, 256 +/- 57; Ni, 1.7 +/- 0.7; Cu, 14 +/- 4; Zn, 122 +/- 27; Cd, 2.8 +/- 0.7; and Pb, 10 +/- 3 ppm/(dry weight). Washing A. marinus cells before analyses was necessary due to interstitial medium within the cell pellets after centrifugation and loose cationic retention by the cells. The principal source of error in the procedure was ascribed to variability due to washing cells with 0.5 M ammonium formate. The mean cationic concentrations for trace elements in the 22 bacterial cultures grown in the basal seawater medium to constant optical density and washed three times with 0.5 M ammonium formate were: Mn, 2.4 +/- 3.8; Fe, 262 +/- 112; Ni, 2.3 +/- 1.8; Cu, 24 +/- 17; Zn, 146 +/- 72; Cd, 3.8 +/- 2.5; and Pb, 17 +/- 21 ppm (dry weight). Major ions were concentrated only occasionally by the cells after washing, whereas Mn, Fe, Ni, Cu, Zn, Cd, and Pb were concentrated from the medium by the following factors on the average: 180, 1,600, 140, 1,200, 750, 1,900, and 900, respectively.
在基础海水培养基中生长后，通过原子吸收分光光度法对22种海洋、河口和陆地来源的细菌进行阳离子含量分析。对海产产碱菌进行了8次独立但重复的测定，得出以下阳离子浓度：钠，5600±2260；镁，1580±740；钾，700±360；钙，790±390；锰，1.7±0.5；铁，256±57；镍，1.7±0.7；铜，14±4；锌，122±27；镉，2.8±0.7；铅，10±3 ppm/（干重）。由于离心后细胞沉淀中的间隙介质以及细胞对阳离子的保留不牢固，分析前对海产产碱菌细胞进行洗涤是必要的。该方法的主要误差来源归因于用0.5 M甲酸铵洗涤细胞导致的变异性。在基础海水培养基中生长至恒定光密度并用0.5 M甲酸铵洗涤三次的22种细菌培养物中，微量元素的平均阳离子浓度为：锰，2.4±3.8；铁，262±112；镍，2.3±1.8；铜，24±17；锌，146±72；镉，3.8±2.5；铅，17±21 ppm（干重）。洗涤后，细胞仅偶尔浓缩主要离子，而锰、铁、镍、铜、锌、镉和铅从培养基中的浓缩平均倍数分别为：180、1600、140、1200、750、1900和900。
