用于研究大环内酯类诱导核糖体甲基化酶产生能力的荧光测定法。
Fluorescence assay for studying the ability of macrolides to induce production of ribosomal methylase.
作者信息
Clarebout Gervais, Leclercq Roland
机构信息
Service de Microbiologie, UPRES EA 2128, Hôpital Côte de Nacre, Université de Caen, 14033 Caen Cedex, France.
出版信息
Antimicrob Agents Chemother. 2002 Jul;46(7):2269-72. doi: 10.1128/AAC.46.7.2269-2272.2002.
Abstract
A screening assay to test the inducing capacity of macrolides by fusing the attenuator of the inducible erm(B) gene from Streptococcus pneumoniae HM28 with the gfpmut1 gene has been designed. Fluorescence was detected under UV light around disks impregnated with inducer macrolides (erythromycin or azithromycin) but not with noninducer ketolides. Induction could be quantified by fluorometry.
摘要
设计了一种筛选试验，通过将肺炎链球菌HM28中可诱导的erm(B)基因的弱化子与gfpmut1基因融合来测试大环内酯类药物的诱导能力。在浸渍有诱导性大环内酯类药物（红霉素或阿奇霉素）的滤纸片周围的紫外光下检测到荧光，而在浸渍有非诱导性酮内酯类药物的滤纸片周围未检测到荧光。诱导作用可以通过荧光测定法定量。
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