Stamouli Maria I, Ferti Angeliki D, Panani Anna D, Raftakis John, Consoli Carla, Raptis Sotirios A, Young Bryan D
Second Department of Internal Medicine Propaedeutic, Athens University, Evangelismos Hospital, Athens, Greece.
Cancer Genet Cytogenet. 2002 May;135(1):23-7. doi: 10.1016/s0165-4608(01)00635-5.
The different genetic alterations observed in diffuse and intestinal types of gastric cancer suggest that these two pathological types may represent different disease entities. We present two cases of primary gastric carcinoma, a well-differentiated intestinal type adenocarcinoma and a poorly differentiated diffuse type adenocarcinoma, both studied by a 24-color multiplex fluorescence in situ hybridization technique (M-FISH). The well-differentiated intestinal type adenocarcinoma exhibited fewer structural abnormalities with five noncomplex translocations, deletions of chromosomes 5q, 6q, and 17q and an i(8q). In the case of poorly differentiated diffuse carcinoma, structural abnormalities predominated and normal homologues were mostly absent. But there were also similarities between the two cases: translocations on 1p and 9p; structural abnormalities of chromosome 8 with consistent loss of 8p; structural abnormalities of 12q; partial loss of chromosome 17 and 18; and polysomy of chromosome 20. This study shows that M-FISH is valuable in identifying hidden structural abnormalities and could, therefore, be useful in the investigation of primary solid tumors.
在弥漫型和肠型胃癌中观察到的不同基因改变表明，这两种病理类型可能代表不同的疾病实体。我们展示了两例原发性胃癌，一例是高分化肠型腺癌，另一例是低分化弥漫型腺癌，均采用24色多重荧光原位杂交技术（M-FISH）进行研究。高分化肠型腺癌表现出较少的结构异常，有5个非复杂易位、5号染色体长臂、6号染色体长臂和17号染色体长臂缺失以及一条8号等臂染色体。在低分化弥漫型癌病例中，结构异常占主导，大多数正常同源染色体缺失。但这两个病例之间也有相似之处：1号染色体短臂和9号染色体短臂易位；8号染色体结构异常，8号染色体短臂持续缺失；12号染色体结构异常；17号和18号染色体部分缺失；以及20号染色体多体性。本研究表明，M-FISH在识别隐藏的结构异常方面具有价值，因此可用于原发性实体瘤的研究。
