Calcagno Danielle-Queiroz, Leal Mariana-Ferreira, Seabra Aline-Damaceno, Khayat Andre-Salim, Chen Elizabeth Suchi, Demachki Samia, Assumpção Paulo Pimentel, Faria Mario Henrique Girão, Rabenhorst Silvia Helena Barem, Ferreira Márcia Valéria Pitombeira, de Arruda Cardoso Smith Marília, Burbano Rommel-Rodríguez
Human Cytogenetics and Toxicological Genetics Laboratory, Department of Biology, Center of Biological Sciences, Federal University of Pará, Belém, PA, Brazil.
World J Gastroenterol. 2006 Oct 14;12(38):6207-11. doi: 10.3748/wjg.v12.i38.6207.
To investigate chromosome 8 numerical aberrations, C-MYC oncogene alterations and its expression in gastric cancer and to correlate these findings with histopathological characteristics of gastric tumors.
Specimens were collected surgically from seven patients with gastric adenocarcinomas. Immunostaining for C-MYC and dual-color fluorescence in situ hybridization (FISH) for C-MYC gene and chromosome 8 centromere were performed.
All the cases showed chromosome 8 aneuploidy and C-MYC amplification, in both the diffuse and intestinal histopathological types of Lauren. No significant difference (P < 0.05) was observed between the level of chromosome 8 ploidy and the site, stage or histological type of the adenocarcinomas. C-MYC high amplification, like homogeneously stained regions (HSRs) and double minutes (DMs), was observed only in the intestinal-type. Structural rearrangement of C-MYC, like translocation, was observed only in the diffuse type. Regarding C-MYC gene, a significant difference (P < 0.05) was observed between the two histological types. The C-MYC protein was expressed in all the studied cases. In the intestinal-type the C-MYC immunoreactivity was localized only in the nucleus and in the diffuse type in the nucleus and cytoplasm.
Distinct patterns of alterations between intestinal and diffuse types of gastric tumors support the hypothesis that these types follow different genetic pathways.
研究胃癌中8号染色体数目畸变、C-MYC癌基因改变及其表达，并将这些结果与胃肿瘤的组织病理学特征相关联。
手术采集7例胃腺癌患者的标本。进行C-MYC免疫染色以及C-MYC基因和8号染色体着丝粒的双色荧光原位杂交（FISH）。
所有病例在Lauren的弥漫型和肠型组织病理学类型中均显示8号染色体非整倍体和C-MYC扩增。8号染色体倍体水平与腺癌的部位、分期或组织学类型之间未观察到显著差异（P<0.05）。仅在肠型中观察到C-MYC高扩增，如均匀染色区（HSRs）和双微体（DMs）。仅在弥漫型中观察到C-MYC的结构重排，如易位。关于C-MYC基因，两种组织学类型之间观察到显著差异（P<0.05）。所有研究病例中均表达C-MYC蛋白。在肠型中，C-MYC免疫反应仅定位于细胞核，而在弥漫型中定位于细胞核和细胞质。
胃肿瘤肠型和弥漫型之间不同的改变模式支持了这些类型遵循不同遗传途径的假说。
