Knott S A, Marklund L, Haley C S, Andersson K, Davies W, Ellegren H, Fredholm M, Hansson I, Hoyheim B, Lundström K, Moller M, Andersson L
Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, United Kingdom.
Genetics. 1998 Jun;149(2):1069-80. doi: 10.1093/genetics/149.2.1069.
A quantitative trait locus (QTL) analysis of growth and fatness data from a three generation pig experiment is presented. The population of 199 F2 animals was derived from a cross between wild boar and Large White pigs. Animals were typed for 240 markers spanning 23 Morgans of 18 autosomes and the X chromosome. A series of analyses are presented within a least squares framework. First, these identify chromosomes containing loci controlling trait variation and subsequently attempt to map QTLs to locations within chromosomes. This population gives evidence for a large QTL affecting back fat and another for abdominal fat segregating on chromosome 4. The best locations for these QTLs are within 4 cM of each other and, hence, this is likely to be a single QTL affecting both traits. The allele inherited from the wild boar causes an increase in fat deposition. A QTL for intestinal length was also located in the same region on chromosome 4 and could be the same QTL with pleiotropic effects. Significant effects, owing to multiple QTLs, for intestinal length were identified on chromosomes 3 and 5. A single QTL affecting growth rate to 30 kg was located on chromosome 13 such that the Large White allele increased early growth rate, another QTL on chromosome 10 affected growth rate from 30 to 70 kg and another on chromosome 4 affected growth rate to 70 kg.
本文呈现了一项对三代猪实验中的生长和脂肪数据进行的数量性状基因座（QTL）分析。199只F2代动物群体源自野猪与大白猪的杂交。对动物进行了跨越18条常染色体和X染色体上23个摩根的240个标记的分型。在最小二乘法框架内进行了一系列分析。首先，这些分析确定了包含控制性状变异基因座的染色体，随后尝试将QTL定位到染色体内的位置。该群体提供了证据，表明存在一个影响背膘的大QTL，以及另一个在4号染色体上分离的腹脂QTL。这些QTL的最佳位置彼此相距4厘摩以内，因此，这可能是一个影响这两个性状的单一QTL。从野猪遗传的等位基因会导致脂肪沉积增加。一个控制肠道长度的QTL也位于4号染色体的同一区域，可能是具有多效性的同一个QTL。在3号和5号染色体上鉴定出了多个QTL对肠道长度产生的显著影响。一个影响体重达30千克时生长速率的单一QTL位于13号染色体上，大白猪的等位基因会提高早期生长速率，10号染色体上的另一个QTL影响体重从30千克到70千克时的生长速率，4号染色体上的另一个QTL影响体重达70千克时的生长速率。
