皮状丝孢酵母和地衣芽孢杆菌共固定用于生化需氧量传感器。

The use of co-immobilization of Trichosporon cutaneum and Bacillus licheniformis for a BOD sensor.

作者信息

Suriyawattanakul L, Surareungchai W, Sritongkam P, Tanticharoen M, Kirtikara K

机构信息

School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Toong-kru, Bangkok 10140, Thailand.

出版信息

Appl Microbiol Biotechnol. 2002 Jun;59(1):40-4. doi: 10.1007/s00253-002-0980-1. Epub 2002 Apr 4.

DOI:10.1007/s00253-002-0980-1

PMID:12073129

Abstract

The microorganisms Trichosporon cutaneum and Bacillus licheniformis were used to develop a microbial biochemical oxygen demand (BOD) sensor. It was found that T. cutaneum gave a greater response to glucose, whereas B. licheniformis gave a better response to glutamic acid. Hence, co-immobilized T. cutaneum and B. licheniformis were used to construct a glucose and glutamic acid sensor with improved sensitivity and dynamic range. A membrane loading of T. cutaneum at 1.1x10(8 )cells ml(-1) cm(-2) and B. licheniformis at 2.2x10(8) cells ml(-1) cm(-2) gave the optimum result: a linear range up to 40 mg BOD l(-1) with a sensitivity of 5.84 nA mg(-1) BOD l. The optimized BOD sensor showed operation stability for 58 intermittent batch measurements, with a standard deviation of 0.0362 and a variance of 0.131 nA. The response time of the co-immobilized microbial BOD sensor was within 5-10 min by steady-state measurement and the detection limit was 0.5 mg BOD l(-1). The BOD sensor was insensitive to pH in the range of pH 6.8-7.2.

摘要

使用微生物皮状丝孢酵母和地衣芽孢杆菌开发了一种微生物生化需氧量（BOD）传感器。发现皮状丝孢酵母对葡萄糖的响应更大，而地衣芽孢杆菌对谷氨酸的响应更好。因此，将皮状丝孢酵母和地衣芽孢杆菌共固定用于构建具有更高灵敏度和动态范围的葡萄糖和谷氨酸传感器。皮状丝孢酵母的膜负载量为1.1×10⁸个细胞·ml⁻¹·cm⁻²，地衣芽孢杆菌的膜负载量为2.2×10⁸个细胞·ml⁻¹·cm⁻²时效果最佳：线性范围高达40 mg BOD·l⁻¹，灵敏度为5.84 nA·mg⁻¹ BOD·l。优化后的BOD传感器在58次间歇分批测量中显示出操作稳定性，标准偏差为0.0362，方差为0.131 nA。通过稳态测量，共固定化微生物BOD传感器的响应时间在5 - 10分钟内，检测限为0.5 mg BOD·l⁻¹。该BOD传感器在pH 6.8 - 7.2范围内对pH不敏感。