Stope M B, Becher D, Hammer E, Schauer F
Institute of Molecular Biology, Friedrich-Loeffler-Institutes, Federal Research Centre for Virus Diseases of Animals, Boddenblick 5a, 17498 Insel Riems, Germany.
Appl Microbiol Biotechnol. 2002 Jun;59(1):62-7. doi: 10.1007/s00253-002-0979-7. Epub 2002 Apr 6.
Thirty-five strains of soil bacteria were grown with biphenyl (BP) and tested for their capacity to cooxidize dibenzofuran (DBF). During metabolism of DBF, the culture medium of 17 strains changed from colorless to orange, indicating a meta-cleavage pathway of DBF degradation. The ring cleavage product of these isolates was shown to be 2-hydroxy-4-(3'-oxo-3' H-benzofuran-2'-yliden)but-2-enoic acid (HOBB). The strain SBUG 271, studied in detail and identified as Rhodococcus erythropolis, degraded DBF via 1,2-dihydroxydibenzofuran. The ensuing meta-cleavage yielded HOBB and salicylic acid. In addition, the four monohydroxylated monomers of DBF and two metabolites, which were not further characterized, were detected. Thus, our results demonstrate that the metabolic mechanism involves lateral dioxygenation of DBF followed by meta-cleavage and occurs in Gram-negative as well as in Gram-positive BP-degrading bacteria.
用联苯（BP）培养了35株土壤细菌，并测试了它们共氧化二苯并呋喃（DBF）的能力。在DBF代谢过程中，17株菌的培养基从无色变为橙色，表明DBF降解存在间位裂解途径。这些菌株的环裂解产物被证明是2-羟基-4-（3'-氧代-3'H-苯并呋喃-2'-亚基）丁-2-烯酸（HOBB）。对菌株SBUG 271进行了详细研究，鉴定为红平红球菌，它通过1,2-二羟基二苯并呋喃降解DBF。随后的间位裂解产生了HOBB和水杨酸。此外，还检测到了DBF的四种单羟基化单体和两种未进一步鉴定的代谢产物。因此，我们的结果表明，代谢机制包括DBF的侧向双加氧作用，随后是间位裂解，并且在革兰氏阴性和革兰氏阳性的BP降解细菌中均会发生。
