Fuse Hiroyuki, Takimura Osamu, Murakami Katsuji, Inoue Hiroyuki, Yamaoka Yukiho
Institute for Marine Resources and Environment, National Institute of Advanced Industrial Science and Technology, 2-2-2 Hirosuehiro, Kure, Hiroshima 737-0197, Japan.
Biosci Biotechnol Biochem. 2003 May;67(5):1121-5. doi: 10.1271/bbb.67.1121.
Marine bacterial strains (BP-PH, CAR-SF, and DBF-MAK) were isolated using biphenyl, carbazole (CAR), or dibenzofuran (DF) respectively as substrates for growth. Their 16S ribosomal DNA sequences showed that the species closest to strain BP-PH, strain CAR-SF, and strain DBF-MAK are Alteromonas macleodii (96.3% identity), Neptunomonas naphthovorans (93.1% identity), and Cycloclasticus pugetii (97.3% identity), respectively. The metabolites produced suggested that strain CAR-SF degrades CAR via dioxygenation in the angular position and by the meta-cleavage pathway, and that strain DBF-MAK degrades DF via both lateral and angular dioxygenation. Polychlorinated biphenyl (KC-300) and 2,3-dichlorodibenzo-p-dioxin were partially degraded by strain BP-PH and strain DBF-MAK, while 2,7-dichlorodibenzo-p-dioxin and 2,4,8-trichlorodibenzofuran remained virtually unchanged.
分别使用联苯、咔唑（CAR）或二苯并呋喃（DF）作为生长底物，分离出海洋细菌菌株（BP-PH、CAR-SF和DBF-MAK）。它们的16S核糖体DNA序列显示，与BP-PH菌株、CAR-SF菌株和DBF-MAK菌株最接近的物种分别是麦克劳德交替单胞菌（同一性为96.3%）、萘食海单胞菌（同一性为93.1%）和普吉特湾环裂菌（同一性为97.3%）。所产生的代谢产物表明，CAR-SF菌株通过角位双加氧作用和间位裂解途径降解咔唑，DBF-MAK菌株通过侧链和角位双加氧作用降解二苯并呋喃。多氯联苯（KC-300）和2,三氯二苯并对二恶英被BP-PH菌株和DBF-MAK菌株部分降解，而2,二氯二苯并对二恶英和2,三氯二苯并呋喃几乎保持不变。
