Hasegawa Miyuki, Amano Ayako, Muraoka Hiroe, Kobayashi Intetsu, Kimoto Mami, Kato Mototsugu, Fujioka Toshio, Nasu Masaru
Chemotherapy Division, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc.
Kansenshogaku Zasshi. 2002 May;76(5):341-6. doi: 10.11150/kansenshogakuzasshi1970.76.341.
We conducted a study for the growth of and selectivity for the desired microorganisms using a newly developed selective culture medium for Helicobacter pylori, Columbia horse blood agar HP (CHBHP), at three different Japanese clinical laboratories, Hokkaido, Kanto and Kyusyu. When standard strains and clinical isolates of H. pylori were examined, the recovery of the organism on the CHBHP media was comparable to that of conventional selective and nonselective media. However, colonies were obviously larger on the CHBHP media. These media yielded the highest H. pylori positive rate for clinical specimens at all the three laboratories. The detection rate of the CHBHP media in H. pylori-positive specimens was higher than that of media commonly used at the three laboratories (98.1% to 100% vs. 88.0% to 96.2%). The CHBHP media also achieved a higher detection rate for specimens from H. pylori-infected animals. CHBHP media have an excellent growth supporting ability and selectivity originating from Columbia agar base and do not require the combined use of non-selective media for the growth and isolation of the organism, resulting in lower cost. Thus, they are useful media for the selective culture and isolation of H. pylori from clinical and animal specimens.
我们在日本的三个不同临床实验室，即北海道、关东和九州，使用新开发的幽门螺杆菌选择性培养基——哥伦比亚马血琼脂HP（CHBHP），对所需微生物的生长和选择性进行了一项研究。当检测幽门螺杆菌的标准菌株和临床分离株时，在CHBHP培养基上该菌的回收率与传统选择性和非选择性培养基相当。然而，在CHBHP培养基上菌落明显更大。在所有这三个实验室中，这些培养基对临床标本产生的幽门螺杆菌阳性率最高。CHBHP培养基在幽门螺杆菌阳性标本中的检测率高于这三个实验室常用的培养基（98.1%至100%对88.0%至96.2%）。CHBHP培养基对来自幽门螺杆菌感染动物的标本也有更高的检测率。CHBHP培养基具有源自哥伦比亚琼脂基础的出色生长支持能力和选择性，并且在该菌的生长和分离方面不需要联合使用非选择性培养基，从而降低了成本。因此，它们是用于从临床和动物标本中选择性培养和分离幽门螺杆菌的有用培养基。
