Hachem C Y, Clarridge J E, Evans D G, Graham D Y
Department of Medicine, Veterans Affairs Medical Center, Houston, Texas 77030, USA.
J Clin Pathol. 1995 Aug;48(8):714-6. doi: 10.1136/jcp.48.8.714.
To determine the best medium for the primary isolation of Helicobacter pylori.
Sixty six gastric mucosal biopsy specimens frozen in 1 ml Cysteine Albimi media with 20% glycerol from 22 histologically proven H pylori infected patients were cultured on brain heart infusion agar (BHIA) with 7% fresh whole defibrinated horse blood, egg yolk agar (EYA), Columbia blood agar-cyclodextrin agar (CBA-Cd), and commercial trypticase soy agar (TSA) supplemented with 5% sheep blood.
Successful primary isolation of H pylori was 96% with BHIA, 78% with TSA, 64% for EYA, and 32% with CBA-Cd. Colonies appeared earlier on BHIA (4.7 +/- 0.1 days, 5.3 +/- 0.4 days, 5.3 +/- 0.4 days, and 7.1 +/- 0.9 days for BHIA, TSA, EYA, and CBA-Cd) and there were more colonies on BHIA than on CBA-Cd, EYA or TSA (599 +/- 88, 104 +/- 66, 260 +/- 107, and 358 +/- 89, respectively).
Success of a medium for passage of isolates apparently does not reliably predict usefulness for primary isolation. Freshly made BHIA with 7% horse blood medium is recommended for primary isolation. However, the easily obtainable TSA media would be the best alternative for routine clinical laboratories with no access to BHIA.
确定用于幽门螺杆菌初次分离的最佳培养基。
从22例经组织学证实感染幽门螺杆菌的患者中获取66份胃黏膜活检标本，标本保存在含有20%甘油的1毫升半胱氨酸白蛋白培养基中，将其接种于含7%新鲜去纤维马血的脑心浸液琼脂（BHIA）、蛋黄琼脂（EYA）、哥伦比亚血琼脂-环糊精琼脂（CBA-Cd）以及添加5%羊血的商业胰蛋白酶大豆琼脂（TSA）上进行培养。
BHIA对幽门螺杆菌的初次分离成功率为96%，TSA为78%，EYA为64%，CBA-Cd为32%。在BHIA上菌落出现得更早（BHIA、TSA、EYA和CBA-Cd分别为4.7±0.1天、5.3±0.4天、5.3±0.4天和7.1±0.9天），且BHIA上的菌落比CBA-Cd、EYA或TSA上的更多（分别为599±88、104±66、260±107和358±89）。
用于菌株传代的培养基的成功与否显然不能可靠地预测其对初次分离的适用性。推荐使用新鲜制备的含7%马血的BHIA培养基进行初次分离。然而，对于无法获取BHIA的常规临床实验室，容易获得的TSA培养基将是最佳选择。
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