Russo Mario Vincenzo
Facoltà di Agraria (DiSTAAM), Università del Molise Via De Sanctis, 86100 Campobasso (CB), Italy.
Ann Chim. 2002 Apr;92(4):469-76.
Lipids were extracted from lemon wax and fractionated into four classes on a silicic acid glass packed column by thin-layer chromatography (TLC). The free fatty acids, the fatty acid composition and the amount of each separated lipids were determined by capillary column gas chromatography (GC). Total lipids (TL) were 60 mg per 100 g raw weight and the ratio of nonpolar lipids (NPLs): glycolipids (GLs): phospholipids (PLs) was about 47:2:2. The main free fatty acids in lemon wax were hexadecanoic acid, cis-9-octadecenoic acid and cis,cis-9,12-octadecadienoic acid, while in the lipid fractions the main fatty acids were hexadecanoic acid in all the fractions, cis-cis-9,12-octadecadienoic and decanoic acids in triglyceride (TG) fraction, dodecanoic and cis-9-octadecenoic acids in diglyceride (DG) fraction and tetradecanoic, octadecanoic and cis-9-octadecenoic acids in GL and PL fractions. The ratio of unsaturated to saturated fatty acids showed a remarkable difference among these four lipid fractions. In PL and GL fractions this ratio was similar, 47.7% and 47.1% respectively, and in TG fraction it was 42.4% while in DG fraction this value was 23.5%.
从柠檬蜡中提取脂质，并通过薄层色谱法（TLC）在硅胶玻璃填充柱上分离为四类。通过毛细管柱气相色谱法（GC）测定游离脂肪酸、脂肪酸组成以及每种分离出的脂质的含量。总脂质（TL）为每100克生重60毫克，非极性脂质（NPLs）：糖脂（GLs）：磷脂（PLs）的比例约为47:2:2。柠檬蜡中的主要游离脂肪酸为十六烷酸、顺式-9-十八碳烯酸和顺式,顺式-9,12-十八碳二烯酸，而在脂质组分中，所有组分中的主要脂肪酸均为十六烷酸，甘油三酯（TG）组分中的主要脂肪酸为顺式,顺式-9,12-十八碳二烯酸和癸酸，甘油二酯（DG）组分中的主要脂肪酸为十二烷酸和顺式-9-十八碳烯酸，糖脂（GL）和磷脂（PL）组分中的主要脂肪酸为十四烷酸、十八烷酸和顺式-9-十八碳烯酸。这四种脂质组分中不饱和脂肪酸与饱和脂肪酸的比例存在显著差异。在磷脂（PL）和糖脂（GL）组分中，该比例相似，分别为47.7%和47.1%，在甘油三酯（TG）组分中为42.4%，而在甘油二酯（DG）组分中该值为23.5%。
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