Zahid Mohammad, Arif Muhammad, Rahman Md Akhlaquer, Singh Kuldeep, Mujahid Mohd
a Herbal Bioactive Research Laboratory, Faculty of Pharmacy , Integral University , Lucknow , India.
J Diet Suppl. 2018 Sep 3;15(5):613-623. doi: 10.1080/19390211.2017.1366388. Epub 2017 Nov 2.
The yield and fatty oil components of the seed kernels of Annona squamosa L. (Family: Annonaceae) were determined by solvent extraction method and gas chromatography-mass spectrometry (GC-MS). Seeds were extracted with ethanol and further fractionated with n-hexane. The free radical-scavenging activities of both ethanolic and n-hexane fraction against 1, 1-diphenyl-2-picrylhydrazyl (DPPH) were determined by UV spectrophotometer at 517 nm. Phytochemical screening revealed the presence of numerous bioactive compounds including steroids, flavonoids, terpenoids, fatty acids, and different types of ester compounds. The prevailing compounds found in ethanolic fraction were n-hexadecanoic acid (10.08%), heptadecene-(8)-carbonic acid-(1) (29.68%), octadecanoic acid (3.61%), 9-octadecenoic acid (Z)-2,3-dihydroxypropyl ester (5.14%), ergost-5-en-3-ol (3.68%), stigmasta-5,22-dien-3-ol (5.93%), and y-sitosterol (8.25%). Compounds found in n-hexane fraction were mainly n-hexadecanoic acid (14.42%), 9,12-octadecadienoic acid (2.87%), cis-vaccenic acid (10.39%), 9-octadecenoic acid (7.03%), hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester (4%), 9-octadecenoic acid (Z)-, 2,3-dihydroxypropyl ester (13.33%), ergost-5-en-3-ol (4.04%), stigmasta-5,22-dien-3-ol, (3.beta.,22e) (6.07%), and y-sitosterol (10.87%). The crude fatty oil was converted into methyl esters and analyzed by GC-MS. Eleven compounds constituting 99.9% of the oil were identified. The presence of saturated and unsaturated fatty acids in ethanolic and n-hexane fraction of A. squamosa seed extract justify the use of this plant to treat many ailments in folk and herbal medicine. Both the fractions have shown significant antioxidant activity. The presence of phenolic compounds and unsaturated fatty acids are reported as possible contributors for antioxidant activity of seed extract.
采用溶剂萃取法和气相色谱 - 质谱联用（GC - MS）法测定了番荔枝（番荔枝科）种子核的得率和脂肪油成分。种子先用乙醇萃取，再用正己烷进一步分离。通过紫外可见分光光度计在517nm处测定乙醇提取物和正己烷提取物对1,1 - 二苯基 - 2 - 苦基肼（DPPH）的自由基清除活性。植物化学筛选显示存在多种生物活性化合物，包括甾体、黄酮类、萜类、脂肪酸和不同类型的酯类化合物。乙醇提取物中主要的化合物有正十六烷酸（10.08%）、十七碳 -（8）- 烯酸 -（1）（29.68%）、十八烷酸（3.61%）、9 - 十八碳烯酸（Z）- 2,3 - 二羟基丙酯（5.14%）、麦角甾 - 5 - 烯 - 3 - 醇（3.68%）、豆甾 - 5,22 - 二烯 - 3 - 醇（5.93%）和γ - 谷甾醇（8.25%）。正己烷提取物中发现的化合物主要有正十六烷酸（14.42%）、9,12 - 十八碳二烯酸（2.87%）、顺式 - vaccenic酸（10.39%）、9 - 十八碳烯酸（7.03%）、十六烷酸2 - 羟基 - 1 -（羟甲基）乙酯（4%）、9 - 十八碳烯酸（Z）- 2,3 - 二羟基丙酯（13.33%）、麦角甾 - 5 - 烯 - 3 - 醇（4.04%）、豆甾 - 5,22 - 二烯 - 3 - 醇（3β,22E）（6.07%）和γ - 谷甾醇（10.87%）。粗脂肪油转化为甲酯后用GC - MS分析。鉴定出占油99.9%的11种化合物。番荔枝种子提取物的乙醇提取物和正己烷提取物中饱和与不饱和脂肪酸的存在证明了该植物在民间和草药医学中用于治疗多种疾病的合理性。两种提取物均表现出显著的抗氧化活性。酚类化合物和不饱和脂肪酸的存在被认为是种子提取物抗氧化活性的可能贡献因素。
