口腔扁平苔藓中的细胞增殖活性及细胞周期调节蛋白的表达
Cell proliferation activity and the expression of cell cycle regulatory proteins in oral lichen planus.
作者信息
Hirota Makoto, Ito Takaaki, Okudela Koji, Kawabe Ryoichi, Yazawa Takuya, Hayashi Hiroyuki, Nakatani Yukio, Fujita Kiyohide, Kitamura Hitoshi
机构信息
Department of Oral and Maxillofacial Surgery, Yokohama City University School of Medicine, Yokohama, Japan.
出版信息
J Oral Pathol Med. 2002 Apr;31(4):204-12. doi: 10.1034/j.1600-0714.2002.310403.x.
BACKGROUND
In oral lichen planus (OLP), destruction of the basal cell layer, which is one of the characteristic histological features, is seen and many changes in cell proliferation, cell repair and cell death occur in the injured mucosal epithelium.
METHODS
We studied mucosal tissues from 19 patients of OLP and 10 controls, with immunohistochemistry for Ki-67, p53, cyclin dependent kinase inhibitors (CDKI) and cyclins. Mitotic count was calculated. TUNEL assay was also performed for evaluation of apoptotic cell death.
RESULTS
Mitotic count, Ki-67 and cyclin D1 labeling indices in the basal and parabasal layers of OLP mucosa were elevated in comparison with those of controls. p53, p21Cip1 and TUNEL indices of OLP mucosa were also increased.
CONCLUSIONS
These complex changes, which concomitantly occur in the injured mucosal epithelium, could contribute to the development and maintenance of characteristic mucosal epithelial architectures seen in OLP.
背景
在口腔扁平苔藓（OLP）中，可见基底细胞层破坏，这是其特征性组织学特征之一，并且在受损的黏膜上皮中会发生细胞增殖、细胞修复和细胞死亡的多种变化。
方法
我们研究了19例OLP患者和10例对照者的黏膜组织，采用免疫组织化学方法检测Ki-67、p53、细胞周期蛋白依赖性激酶抑制剂（CDKI）和细胞周期蛋白。计算有丝分裂计数。还进行了TUNEL检测以评估凋亡细胞死亡。
结果
与对照组相比，OLP黏膜基底和副基底层的有丝分裂计数、Ki-67和细胞周期蛋白D1标记指数升高。OLP黏膜的p53、p21Cip1和TUNEL指数也增加。
结论
这些在受损黏膜上皮中同时发生的复杂变化，可能有助于OLP中所见特征性黏膜上皮结构的形成和维持。
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