Shiva Atena, Zamanian Ali, Arab Shahin, Boloki Mahsa
Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran.
Dept. of Restorative Dentistry, School of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran.
J Dent (Shiraz). 2018 Jun;19(2):118-123.
Oral lichen planus is a common mucocutaneous lesion with a chronic inflammatory process mediated by immune factors while a few cases of the disease become malignant.
This study aimed to determine the frequency of p53 marker as a tumor suppressor in patients with erosive and non-erosive oral lichen planus (OLP) by using immunohistochemical methods.
This descriptive cross-sectional study investigated the p53 expression in 16 erosive OLP, 16 non-erosive OLP samples, and 8 samples of normal oral mucosa through immunohistochemistry. The percentage of stained cells in basal and suprabasal layers, and inflammatory infiltrate were graded according to the degree of staining; if 0%, <10%, 10-25%, and >50% of the cells were stained, they were considered as (-), (+), (++), (+++) and (++++), respectively. The obtained data was statistically analyzed and compared by using Chi square and Fisher's exact test.
The mean percentage of p53 positive cells in erosive OLP (34.5±14.2) was considerably higher than that in non-erosive OLP (23.8±10.4) and normal mucosa (17.5±17). There was a significant difference among the three groups of erosive, non-erosive and control in terms of staining intensity. No significant difference existed between the patients' age and sex in the two OLP groups.
The increased incidence of p53 from normal mucosa to erosive OLP indicated the difference between biological behavior of erosive and non-erosive OLP. It can be claimed that the erosive OLP has great premalignant potential compared with the non-erosive one.
口腔扁平苔藓是一种常见的黏膜皮肤病变，由免疫因素介导慢性炎症过程，少数病例会发生恶变。
本研究旨在通过免疫组织化学方法确定p53作为肿瘤抑制因子在糜烂性和非糜烂性口腔扁平苔藓（OLP）患者中的表达频率。
本描述性横断面研究通过免疫组织化学研究了16例糜烂性OLP、16例非糜烂性OLP样本以及8例正常口腔黏膜样本中p53的表达情况。根据染色程度对基底层和基底上层的染色细胞百分比以及炎症浸润进行分级；若0%、<10%、10 - 25%和>50%的细胞被染色，则分别视为（-）、（+）、（++）、（+++）和（++++）。使用卡方检验和费舍尔精确检验对所得数据进行统计学分析和比较。
糜烂性OLP中p53阳性细胞的平均百分比（34.5±14.2）显著高于非糜烂性OLP（23.8±10.4）和正常黏膜（17.5±17）。糜烂性、非糜烂性和对照组三组在染色强度方面存在显著差异。两个OLP组患者的年龄和性别之间无显著差异。
从正常黏膜到糜烂性OLP，p53发生率的增加表明糜烂性和非糜烂性OLP生物学行为存在差异。可以认为，与非糜烂性OLP相比，糜烂性OLP具有更大的恶变潜能。
