Kao Shou-Yen, Tu Hsi-Feng, Chang Kuo-Wei, Chang Che-Shoa, Yang Cheng-Chei, Lin Shu-Chun
Department of Dentistry, Oral Maxillofacial Surgery, Veterans General Hospital-Taipei/School of Dentistry, National Yang-Ming University, Taipei, Taiwan, R.O.C.
J Oral Pathol Med. 2002 Apr;31(4):220-6. doi: 10.1034/j.1600-0714.2002.310405.x.
Within the abundant retinoids nuclear receptors, abnormally low expression of the RAR-beta has been shown to contribute to neoplastic progression in oral epithelium in western countries. Distinctly different risk factors contributing to oral squamous cell carcinoma (OSCC) in epidemiologically different societies denote the value of exploring the role of RAR-beta expression in OSCC associated with betel quid (BQ) use in our society.
We examined the cellular expression of RAR-beta using in situ hybridization (ISH) analysis on 38 pairs of surgical specimens of primary OSCC and non-cancerous matched tissues (NCMT) to correlate with their clinico-pathological features including age, sites of tumor, habit of BQ use, stage, size of primary tumor, lymph node metastasis, differentiation.
Of all cases analyzed, BQ users were significantly younger than non-BQ users (51.2 +/- 2.1 vs. 60.2 +/- 2.6, P = 0.01). 52% OSCC of BQ users (13/25) and 23% OSCC of non-BQ users (3/13) exhibited the absence of RAR-beta expression. In 17 paired-samples from buccal mucosa (BM), most NCMT and less than half of OSCC exhibited RAR-beta expression (16/17, 94% vs. 8/17, 47%, P = 0.003). The RAR-beta expression was seen in the vast majority of the well-differentiated OSCC and in less than half of the moderately differentiated OSCC only (15/20, 75% vs. 7/18, 39%, P = 0.03).
A correlation between the loss of RAR-beta expression and more advanced histopathological grade tumors was observed. This study also suggests that the loss of RAR-beta expression is significant in BM OSCC, which preferentially occurs in BQ users.
在众多类视黄醇核受体中，已表明视黄酸受体β（RAR-β）表达异常降低会促使西方国家口腔上皮发生肿瘤进展。在流行病学特征不同的社会中，导致口腔鳞状细胞癌（OSCC）的危险因素明显不同，这表明在我们的社会中探索RAR-β表达在与嚼槟榔（BQ）相关的OSCC中的作用具有重要意义。
我们采用原位杂交（ISH）分析技术，对38对原发性OSCC手术标本及其癌旁匹配组织（NCMT）进行RAR-β细胞表达检测，并将其与临床病理特征（包括年龄、肿瘤部位、嚼槟榔习惯、分期、原发肿瘤大小、淋巴结转移、分化程度）进行关联分析。
在所有分析病例中，嚼槟榔者明显比不嚼槟榔者年轻（51.2±2.1岁对60.2±2.6岁，P = 0.01）。嚼槟榔者的OSCC中有52%（13/25）、不嚼槟榔者的OSCC中有23%（3/13）表现为RAR-β表达缺失。在来自颊黏膜（BM）的17对样本中，大多数NCMT及不到一半的OSCC表现出RAR-β表达（16/17，94%对8/17，47%，P = 0.003）。RAR-β表达仅在绝大多数高分化OSCC中可见，而在中分化OSCC中不到一半（15/20，75%对7/18，39%，P = 0.03）。
观察到RAR-β表达缺失与组织病理学分级更高的肿瘤之间存在相关性。本研究还表明，RAR-β表达缺失在BM OSCC中具有重要意义，且BM OSCC在嚼槟榔者中更为常见。
