Gyula K
Morphol Igazsagugyi Orv Sz. 1975 Jul;15(3):218-20.
Chinacrine fluorescence of the Y chromatin in histological sections is a useful tool for determination of nuclear sex. Nuclei of fibroblasts and smooth muscle cells seems to be most suitable for this investigation. Tissue specimens should be fixed for 5 minutes in hot formalin. Frequency of the Y chromatin in frozen sections of male origin was found to be 38--70 per cent, in paraffin embedded sections 28--44 per cent. It seems likely, that paraffin decreases chinacrin binding capacity of the Y chromatin. Frequency of the Barr-body in tissue specimens of female origin was found to be similar both in frozen and paraffin embedded sections. For the investigation of X and Y chromatin in tissue sections the technique of frozen sections should be used.
组织学切片中Y染色质的阿的平荧光是确定核性别的有用工具。成纤维细胞和平滑肌细胞的细胞核似乎最适合这项研究。组织标本应在热福尔马林中固定5分钟。发现男性来源冰冻切片中Y染色质的频率为38% - 70%，石蜡包埋切片中为28% - 44%。石蜡似乎可能降低Y染色质的阿的平结合能力。在女性来源的组织标本中，冰冻切片和石蜡包埋切片中巴氏小体的频率相似。对于组织切片中X和Y染色质的研究，应采用冰冻切片技术。
