Schmechta H, Porstmann T
Zentralbl Allg Pathol. 1976;120(1):61-7.
Frequency of detectable Y-bodies in interphase nuclei after mepacrin staining of paraffin embedded formalin-fixed tissue was compared with those of cryostat sections of the native material and organ smears with and without formalin fixing. In formalin fixed smears from males 42--70% of investigated cells showed the Y-body. Sections of formalin-fixed and paraffin embedded male organs demonstrated in 12--24% of their cells the typical fluorescence for the Y-chromatin. High significantly less fluorescent Y-like bodies were found in smears and sections of all proofed organs from women prepared in the same manner. Mepacrin staining in combination with investigation of Barr-bodies permits a fast determination of sex of both organ smears and sections, too.
将甲吖啶染色后的石蜡包埋福尔马林固定组织的间期核中可检测到的Y小体频率，与天然材料的冷冻切片以及有无福尔马林固定的器官涂片的Y小体频率进行了比较。在男性福尔马林固定涂片中，42%-70%的被研究细胞显示出Y小体。福尔马林固定并石蜡包埋的男性器官切片中，12%-24%的细胞显示出Y染色质的典型荧光。以同样方式制备的所有经检测女性器官的涂片和切片中，发现的荧光Y样小体明显少得多。甲吖啶染色与巴氏小体检测相结合，也能够快速确定器官涂片和切片的性别。
