Nguyen-Legros J, Berger B, Alvarez C
C R Seances Acad Sci D. 1979 Dec 17;289(16):1311-4.
A new radioautographic technique for the visualization of central dopaminergic fibers is described. This technique is based upon in vitro uptake of triatiated norepinephrine or tritiated dopamine in the presence of monoamine oxidase inhibitor and specific uptake inhibitor in noradrenergic fibers. After a short step in glutaraldehyde, the use of osmium vapours as a fixative, instead of osmic acid immersion, allows a satisfactory retention of triatiated amines within dopaminergic fibers.
本文描述了一种用于可视化中枢多巴胺能纤维的新放射自显影技术。该技术基于在单胺氧化酶抑制剂和去甲肾上腺素能纤维中的特异性摄取抑制剂存在的情况下，体外摄取三碘化去甲肾上腺素或三碘化多巴胺。在经过戊二醛的短暂处理后，使用锇蒸气作为固定剂，而不是锇酸浸泡，能够使三碘化胺在多巴胺能纤维中得到令人满意的保留。
