Nguyen-Legros J, Berger B, Alvarez C
Brain Res. 1981 Jun 1;213(2):265-76. doi: 10.1016/0006-8993(81)90233-x.
An original method is reported for the radioautographic visualizing of dopaminergic axons terminals in the rat central nervous system. This method is based on an in vitro labelling with either tritiated norepinephrine or dopamine under conditions of specificity previously established by fluorescence histochemistry. The immobilization of [3H]catecholamines in their sites of uptake and storage during post-fixation for electron microscopic processing was ensured by the use of osmic acid vapours instead of osmic acid solution. Selective labelling of the dopaminergic fibers may be achieved throughout large brain sections and the technique should be applicable to human biopsy material.
报道了一种用于大鼠中枢神经系统中多巴胺能轴突终末放射自显影可视化的原创方法。该方法基于在荧光组织化学先前确立的特异性条件下，用氚标记的去甲肾上腺素或多巴胺进行体外标记。通过使用锇酸蒸汽而非锇酸溶液，确保了在电子显微镜处理的后固定过程中，[3H]儿茶酚胺固定在其摄取和储存位点。多巴胺能纤维的选择性标记可在整个大脑大切片中实现，并且该技术应适用于人类活检材料。
