Rzeszowska-Wolny J, Palyvoda O, Polanska J, Wygoda A, Hancock R
Department of Experimental and Clinical Radiobiology, M. Sklodowska-Curie Memorial Cancer Center and Institute, Wybrze_ze Armii Krajowej 15, 44-100 Gliwice, Poland.
Int J Radiat Biol. 2008 Aug;84(8):635-42. doi: 10.1080/09553000802087041.
To study the relationship between lymphocyte radiosensitivity measured in vitro and acute reactions to radiotherapy in patients with head and neck cancer.
Acute reactions were measured in 34 patients using the Dische scale. Lymphocyte radiosensitivity was measured using the alkaline comet assay, the micronucleus assay, the nuclear division index and morphological assessment of apoptosis.
There was a weak, statistically significant correlation between in vitro radiosensitivity measured as the rate of DNA damage repair and the cumulative radiation dose exerting the maximum acute reaction scored (r = -0.366, p = 0.039, n = 34). Subgroup analyses showed that for patients with a low level of radiation-induced DNA damage there was a statistically significant relationship between lymphocyte radiosensitivity measured as inhibition of proliferation and acute toxicity (r = -0.621, p = 0.007, n = 18). For patients with a high level of residual DNA damage, there was a relationship between lymphocyte radiosensitivity measured using the micronucleus assay and acute toxicity (r = -0.597, p = 0.023, n = 14).
Combining two measures of radiosensitivity improves the ability to correlate in vitro lymphocyte radiosensitivity and acute radiotherapy toxicity data.
研究体外测量的淋巴细胞放射敏感性与头颈癌患者放疗急性反应之间的关系。
采用迪施量表对34例患者的急性反应进行测量。使用碱性彗星试验、微核试验、核分裂指数和凋亡形态学评估来测量淋巴细胞放射敏感性。
以DNA损伤修复率衡量的体外放射敏感性与产生最大急性反应评分的累积辐射剂量之间存在微弱的、具有统计学意义的相关性（r = -0.366，p = 0.039，n = 34）。亚组分析表明，对于辐射诱导DNA损伤水平较低的患者，以增殖抑制衡量的淋巴细胞放射敏感性与急性毒性之间存在统计学意义的关系（r = -0.621，p = 0.007，n = 18）。对于残留DNA损伤水平较高的患者，使用微核试验测量的淋巴细胞放射敏感性与急性毒性之间存在关系（r = -0.597，p = 0.023，n = 14）。
结合两种放射敏感性测量方法可提高将体外淋巴细胞放射敏感性与急性放疗毒性数据相关联的能力。
