Jern Patric, Lindeskog Mats, Karlsson Damita, Blomberg Jonas
Section of Virology, Department of Medical Science, Uppsala University, SE-751 85 Uppsala, Sweden.
AIDS Res Hum Retroviruses. 2002 Jun 10;18(9):671-6. doi: 10.1089/088922202760019383.
Human endogenous retroviruses (HERVs) are estimated to represent at least 1% of the human genome. An HERV-H env SU sequence (HERV-H19) was used to screen the high-throughput (htgs) and nonredundant (nr) databases for other HERV-H SU open reading frames (ORFs) and thus possible functional proteins. Using PCR with primers derived from HERV-H19 SU, we also obtained several new sequences with ORFs from a human DNA sample. In a phylogenetic analysis, ORF-containing sequences clustered with HERV-H sequences from chromosomes 1 and 2. SU ORF- and non-SU ORF-containing elements had about the same difference between 5' and 3' long terminal repeats (LTRs) (about 4%), indicating a similar time of integration. SU ORF sequences had a moderately high number of synonymous-versus-nonsynonymous mutations, which indicates a selection for maintenance of the HERV-H SU ORFs.
据估计，人类内源性逆转录病毒（HERV）至少占人类基因组的1%。使用一个HERV-H env SU序列（HERV-H19）在高通量（htgs）和非冗余（nr）数据库中筛选其他HERV-H SU开放阅读框（ORF），从而寻找可能的功能蛋白。利用从HERV-H19 SU衍生的引物进行PCR，我们还从一份人类DNA样本中获得了几个带有ORF的新序列。在系统发育分析中，含有ORF的序列与来自1号和2号染色体的HERV-H序列聚类。含有SU ORF和不含SU ORF的元件在5'和3'长末端重复序列（LTR）之间的差异大致相同（约4%），这表明整合时间相似。SU ORF序列具有中等数量的同义突变与非同义突变，这表明对HERV-H SU ORF的维持存在选择。
