Tsen Hau-Yang, Lin Jer-Sheng, Hsih Hsien-Yee
Department of Food Science, National Chung-Hsing University, 250 Kuokuang Road, ROC, Taichung, Taiwan.
Vet Microbiol. 2002 Jun 5;87(1):73-80. doi: 10.1016/s0378-1135(02)00020-2.
Salmonella enterica subspecies enterica serovar Typhimurium is a common pathogen for humans and animals. In order to trace the clonal relationship and to find the circulating strains between human and animal isolates, chromosomal DNAs from 87 serovar Typhimurium strains isolated from animals (pigs were the majority) were subjected to XbaI and SpeI digestion and pulsed field gel electrophoresis (PFGE). For the 87 animal isolates, 38 PFGE pattern combinations were obtained. As the subtyping results from animal isolates were compared with those from the 45 human isolates, it was found that 14 of the animal isolates and 13 of the human isolates shared a common PFGE pattern combination, i.e., pattern XgSf (or called X5S4). When these human and animal isolates were subjected to antibiotic susceptibility test using 11 antibiotics, it was found that strains of pattern XgSf (X5S4) belong to a common antibiogram pattern which is tetracycline, gentamicin, ampicillin, streptomycin and chloramphenicol resistant. Since most of the animal and human strains in pattern XgSf were originally isolated from various areas over different years, strains of this PFGE pattern may be the most epidemic strains which circulating between human and animal sources.
肠炎沙门氏菌肠炎亚种鼠伤寒血清型是人和动物的常见病原体。为了追踪克隆关系并找出人和动物分离株之间的流行菌株，对从动物（大多数是猪）分离的87株鼠伤寒血清型菌株的染色体DNA进行了XbaI和SpeI酶切以及脉冲场凝胶电泳（PFGE）分析。对于这87株动物分离株，获得了38种PFGE图谱组合。将动物分离株的亚型分析结果与45株人类分离株的结果进行比较时，发现14株动物分离株和13株人类分离株共享一种常见的PFGE图谱组合，即XgSf型（或称为X5S4型）。当使用11种抗生素对这些人和动物分离株进行药敏试验时，发现XgSf型（X5S4型）菌株属于一种常见的抗菌谱类型，即对四环素、庆大霉素、氨苄青霉素、链霉素和氯霉素耐药。由于XgSf型的大多数动物和人类菌株最初是在不同年份从不同地区分离的，这种PFGE图谱类型的菌株可能是在人和动物来源之间传播的最流行菌株。
