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关于单核苷酸多态性(SNP)及其他类型分子标记及其在动物遗传学中的应用的综述。

A review on SNP and other types of molecular markers and their use in animal genetics.

作者信息

Vignal Alain, Milan Denis, SanCristobal Magali, Eggen André

机构信息

Laboratoire de génétique cellulaire, Inra, chemin de Borde-Rouge, Auzeville BP 27, 31326 Castanet-Tolosan cedex, France.

出版信息

Genet Sel Evol. 2002 May-Jun;34(3):275-305. doi: 10.1186/1297-9686-34-3-275.

Abstract

During the last ten years, the use of molecular markers, revealing polymorphism at the DNA level, has been playing an increasing part in animal genetics studies. Amongst others, the microsatellite DNA marker has been the most widely used, due to its easy use by simple PCR, followed by a denaturing gel electrophoresis for allele size determination, and to the high degree of information provided by its large number of alleles per locus. Despite this, a new marker type, named SNP, for Single Nucleotide Polymorphism, is now on the scene and has gained high popularity, even though it is only a bi-allelic type of marker. In this review, we will discuss the reasons for this apparent step backwards, and the pertinence of the use of SNPs in animal genetics, in comparison with other marker types.

摘要

在过去十年中,揭示DNA水平多态性的分子标记在动物遗传学研究中发挥着越来越重要的作用。其中,微卫星DNA标记因其通过简单的聚合酶链反应(PCR)易于使用,随后通过变性凝胶电泳确定等位基因大小,以及每个位点的大量等位基因所提供的高度信息性而被最广泛使用。尽管如此,一种名为单核苷酸多态性(SNP)的新型标记现在已经出现并广受欢迎,尽管它只是一种双等位基因类型的标记。在这篇综述中,我们将讨论这种明显倒退的原因,以及与其他标记类型相比,SNP在动物遗传学中使用的相关性。

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