Zhou Jiaqi, Wei Jie, Wang Hong, Li Huan, Zhao Lan, Fu Rui, Yue Bingfei
Division of Laboratory Animal Monitoring, National Institutes for Food and Drug Control, Beijing 102629, China.
R&D Center, Beijing Minhai Biotechnology Co., Ltd., Beijing 102629, China.
Genes (Basel). 2024 Dec 19;15(12):1622. doi: 10.3390/genes15121622.
The genetic quality of laboratory mice may have a direct impact on the results of research. Therefore, it is essential to improve genetic monitoring methods to guarantee research quality. However, few current methods boast high efficiency, high throughput, low cost, and general applicability at the same time. First, we got 34 SNP loci from previous studies for inbred strains and screened out 15 loci with good polymorphism for outbred groups from these 34 loci. Then, by using the Luminex xTAG assay, we tested inbred strains and outbred groups. We tested commonly used inbred strains and five DNA samples from the International Council for Laboratory Animal Science, obtaining correct genotyping results. Additionally, some loci were potentially confirmed to be useful for distinguishing C57BL/6 and BALB/c mouse substrains. Furthermore, we tested three outbred groups and analyzed the genetic structure, and we compared the results of the SNP markers by xTAG assay to the STR markers by PCR, the trends of the three groups are the same. In our studies, the panels could meet the requirements for method promotion and provide a good choice for the genetic monitoring of inbred and outbred mice.
实验小鼠的遗传质量可能会对研究结果产生直接影响。因此，改进遗传监测方法以确保研究质量至关重要。然而，目前很少有方法能同时具备高效、高通量、低成本和广泛适用性。首先，我们从先前针对近交系的研究中获取了34个单核苷酸多态性（SNP）位点，并从这34个位点中筛选出15个具有良好多态性的位点用于远交群体。然后，通过使用Luminex xTAG检测法，我们对近交系和远交群体进行了检测。我们检测了常用的近交系以及来自国际实验动物科学理事会的五个DNA样本，获得了正确的基因分型结果。此外，一些位点有可能被证实可用于区分C57BL/6和BALB/c小鼠亚系。此外，我们检测了三个远交群体并分析了其遗传结构，还将xTAG检测法的SNP标记结果与PCR的STR标记结果进行了比较，三组的趋势相同。在我们的研究中，这些检测板能够满足方法推广的要求，并为近交和远交小鼠的遗传监测提供了一个良好的选择。
