S-腺苷同型半胱氨酸对大鼠肝脏中三种纯化的tRNA甲基转移酶的抑制作用。
S-adenosylhomocysteine inhibition of three purified tRNA methyltransferases from rat liver.
作者信息
Glick J M, Ross S, Leboy P S
出版信息
Nucleic Acids Res. 1975 Oct;2(10):1639-51. doi: 10.1093/nar/2.10.1639.
Abstract
Three tRNA methyltransferases from rat liver have been fractionated and purified greater than 100-fold. These enzymes have been examined for their sensitivity to inhibition by S-adenosylhomocysteine (SAH). The methyltransferase which forms m2-guanine in the region between the dihydrouridine loop and the acceptor stem of tRNA (m2-guanine methyltransferase I) is least sensitive to SAH inhibition, with a Ki of 8 muM. The enzyme responsible for forming m2-guanine between the dihydrouridine and anticodon loops (m2-guanine methyltransferase II) has a Ki of 0.3 muM, while m1-adenine methyltransferase shows intermediate sensitivity to SAH (Ki = 2.4 muM). All three methyltransferases have similar Km's for the S-adenosylmethionine substrate (1.5-2.0 muM). These results are consistent with the hypothesis that activity of individual tRNA methyltransferases may be controlled by enzyme systems which alter cellular SAH levels.
摘要
已从大鼠肝脏中分离并纯化出三种tRNA甲基转移酶，纯化倍数超过100倍。已检测这些酶对S-腺苷同型半胱氨酸（SAH）抑制作用的敏感性。在tRNA的二氢尿嘧啶环和受体茎之间区域形成m2-鸟嘌呤的甲基转移酶（m2-鸟嘌呤甲基转移酶I）对SAH抑制最不敏感，其Ki为8μM。负责在二氢尿嘧啶环和反密码子环之间形成m2-鸟嘌呤的酶（m2-鸟嘌呤甲基转移酶II）的Ki为0.3μM，而m1-腺嘌呤甲基转移酶对SAH表现出中等敏感性（Ki = 2.4μM）。所有三种甲基转移酶对S-腺苷甲硫氨酸底物的Km值相似（1.5 - 2.0μM）。这些结果与以下假设一致，即单个tRNA甲基转移酶的活性可能受改变细胞SAH水平的酶系统控制。
相似文献
1
S-adenosylhomocysteine inhibition of three purified tRNA methyltransferases from rat liver.S-腺苷同型半胱氨酸对大鼠肝脏中三种纯化的tRNA甲基转移酶的抑制作用。
Nucleic Acids Res. 1975 Oct;2(10):1639-51. doi: 10.1093/nar/2.10.1639.
2
S-adenosylhomocysteine analogues as inhibitors of specific tRNA methylation.S-腺苷同型半胱氨酸类似物作为特定tRNA甲基化的抑制剂
Biochim Biophys Acta. 1978 Aug 23;520(1):153-63. doi: 10.1016/0005-2787(78)90016-3.
3
Purification and characterization of two tRNA-(guanine)-methyltransferases from rat liver.大鼠肝脏中两种tRNA（鸟嘌呤）甲基转移酶的纯化与特性分析
Biochim Biophys Acta. 1978 Mar 29;518(1):158-71. doi: 10.1016/0005-2787(78)90125-9.
4
Inhibition of Newcastle disease virion messenger RNA (guanine-7-)-methyltransferase by analogues of S-adenosylhomocysteine.S-腺苷高半胱氨酸类似物对新城疫病毒粒子信使核糖核酸（鸟嘌呤-7-）-甲基转移酶的抑制作用
Biochemistry. 1977 Aug 23;16(17):3928-32. doi: 10.1021/bi00636a032.
5
[New studies on inhibition of tRNA N2 guanine methyltransferase by S-adenosyl-homocysteine and S-adenosyl-methionine analogs].[S-腺苷同型半胱氨酸和S-腺苷甲硫氨酸类似物对tRNA N2鸟嘌呤甲基转移酶抑制作用的新研究]
Biochimie. 1976;58(1-2):201-5. doi: 10.1016/s0300-9084(76)80370-7.
6
Escherichia coli tRNA (uracil-5-)-methyltransferase: Inhibition by analogues of adenosylhomocysteine.大肠杆菌tRNA（尿嘧啶-5-）-甲基转移酶：腺苷同型半胱氨酸类似物的抑制作用。
Enzyme. 1979;24(6):353-7. doi: 10.1159/000458689.
7
tRNA methyltransferases from rat liver. Differences in response of partially purified enzymes to polyamines and inorganic salts.来自大鼠肝脏的tRNA甲基转移酶。部分纯化的酶对多胺和无机盐反应的差异。
Biochim Biophys Acta. 1976 Jun 2;435(1):30-8. doi: 10.1016/0005-2787(76)90188-x.
8
Purification and properties of tRNA(adenine-1)-methyltransferase from rat liver.大鼠肝脏tRNA（腺嘌呤-1）-甲基转移酶的纯化及性质
J Biol Chem. 1977 Jul 25;252(14):4790-5.
9
Effect of S-adenosylhomocysteine on sulfhydryl xenobiotic transmethylases in rat liver.S-腺苷同型半胱氨酸对大鼠肝脏巯基外源性物质转甲基酶的影响。
Biochem Biophys Res Commun. 1985 Apr 30;128(2):965-71. doi: 10.1016/0006-291x(85)90141-x.
10
Analogs of S-adenosylhomocysteine as potential inhibitors of biological transmethylation. Inhibition of several methylases by S-tubercidinylhomocysteine.S-腺苷高半胱氨酸类似物作为生物转甲基作用的潜在抑制剂。S-杀结核菌素基高半胱氨酸对几种甲基化酶的抑制作用。
J Med Chem. 1974 Dec;17(12):1286-9. doi: 10.1021/jm00258a011.
引用本文的文献
1
The Contribution of Homocysteine Metabolism Disruption to Endothelial Dysfunction: State-of-the-Art.同型半胱氨酸代谢紊乱对血管内皮功能障碍的影响：最新研究进展。
Int J Mol Sci. 2019 Feb 17;20(4):867. doi: 10.3390/ijms20040867.
2
S-Adenosyl-L-homocysteine in brain : Regional concentrations, catabolism, and the effects of methionine sulfoximine.脑中 S-腺苷-L-高半胱氨酸：区域浓度、分解代谢以及甲硫氨酸亚砜imine 的影响。
Neurochem Res. 1977 Feb;2(1):27-38. doi: 10.1007/BF00966019.
3
The developmental pattern of homologous and heterologous tRNA methylation in rat brain differential effect of spermidine.大鼠脑中同源和异源 tRNA 甲基化的发育模式。精脒的差异效应。
Neurochem Res. 1976 Aug;1(4):369-84. doi: 10.1007/BF00966229.
4
Interplay between cellular methyl metabolism and adaptive efflux during oncogenic transformation from chronic arsenic exposure in human cells.人类细胞长期砷暴露致癌转化过程中细胞甲基代谢与适应性外排之间的相互作用。
J Biol Chem. 2008 Jul 11;283(28):19342-50. doi: 10.1074/jbc.M802942200. Epub 2008 May 16.
5
Synthesis of autoinducer 2 by the lyme disease spirochete, Borrelia burgdorferi.莱姆病螺旋体伯氏疏螺旋体对自诱导物2的合成。
J Bacteriol. 2005 May;187(9):3079-87. doi: 10.1128/JB.187.9.3079-3087.2005.
6
Effects of DL-ethionine on mouse liver tRNA base composition.DL-乙硫氨酸对小鼠肝脏转运核糖核酸碱基组成的影响。
Nucleic Acids Res. 1976 Sep;3(9):2243-53. doi: 10.1093/nar/3.9.2243.
7
Purification and characterization of a tRNA methylase from Salmonella typhimurium.鼠伤寒沙门氏菌tRNA甲基化酶的纯化与特性分析
J Bacteriol. 1978 Oct;136(1):191-200. doi: 10.1128/jb.136.1.191-200.1978.
8
S-adenosylhomocysteine toxicity in normal and adenosine kinase-deficient lymphoblasts of human origin.人源正常及腺苷激酶缺陷淋巴母细胞中的S-腺苷同型半胱氨酸毒性
Proc Natl Acad Sci U S A. 1979 May;76(5):2450-4. doi: 10.1073/pnas.76.5.2450.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Influence of polyamines and salts on changing patterns of tRNA methylation.多胺和盐对tRNA甲基化变化模式的影响。
FEBS Lett. 1971 Aug 1;16(2):117-120. doi: 10.1016/0014-5793(71)80347-2.
3
Studies on inhibitors of mammalian tRNA methylases.哺乳动物tRNA甲基化酶抑制剂的研究。
FEBS Lett. 1971 Jul 15;16(1):13-16. doi: 10.1016/0014-5793(71)80672-5.
4
N2-guanine specific transfer RNA methyltransferase II from rat liver.来自大鼠肝脏的N2-鸟嘌呤特异性转移RNA甲基转移酶II
Nucleic Acids Res. 1974 Nov;1(11):1479-96. doi: 10.1093/nar/1.11.1479.
5
N2-guanine specific transfer RNA methyltransferase I from rat liver and leukemic rat spleen.来自大鼠肝脏和白血病大鼠脾脏的N2-鸟嘌呤特异性转移RNA甲基转移酶I
Nucleic Acids Res. 1974 Nov;1(11):1455-78. doi: 10.1093/nar/1.11.1455.
6
Nicotinamide: a natural inhibitor of tRNA methylase.烟酰胺：一种天然的tRNA甲基化酶抑制剂。
Biochem Biophys Res Commun. 1971 Feb 19;42(4):602-7. doi: 10.1016/0006-291x(71)90530-4.
7
Stimulation of soluble ribonucleic acid methylase activity by polyamines.多胺对可溶性核糖核酸甲基酶活性的刺激作用。
Biochemistry. 1970 Mar 31;9(7):1577-84. doi: 10.1021/bi00809a016.
8
Nucleotide sequence specificities of guanylate residue-specific tRNA methylases from rat liver.大鼠肝脏中鸟苷酸残基特异性tRNA甲基化酶的核苷酸序列特异性
Biochem Biophys Res Commun. 1970 Jul 27;40(2):306-13. doi: 10.1016/0006-291x(70)91010-7.
9
Purification and characterization of glycine N-methyltransferase.甘氨酸N-甲基转移酶的纯化与特性分析
J Biol Chem. 1973 Jan 10;248(1):69-72.
10
Methylation of Escherichia coli transfer ribonucleic acids by adenylate residue-specific transfer ribonucleic acid methylase from rat liver.大鼠肝脏腺苷酸残基特异性转移核糖核酸甲基酶对大肠杆菌转移核糖核酸的甲基化作用
Biochemistry. 1974 Aug 27;13(18):3683-8. doi: 10.1021/bi00715a010.
Zotero 插件