Cerrutti Jorgelina A, Brandoni Anabel, Quaglia Nora B, Torres Adriana M
Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, CONICET, Argentina.
Mol Cell Biochem. 2002 Apr;233(1-2):175-9. doi: 10.1023/a:1015563021602.
Sex differences in the kinetic parameters of p-aminohippuric acid (PAH) transport in brush border (BBMV) and basolateral (BLMV) membrane vesicles from kidney cortex have been observed. Membrane fluidity of BBMV was higher in females as compared with male rats as indicated by anisotropy values (0.1897 +/- 0.0010 vs. 0.2003 +/- 0.0014, p < 0.05, for females and males respectively). Membrane fluidity of BLMV were similar in both sexes. Western blot studies revealed that OAT1 protein in female BLMV was present at only 40% of level found in BLMV from male rats. The lower expression of OAT1 in BLMV in association with the higher BBMV fluidity (which may affect the affinity of PAH transporter in this membrane domain) observed in females may be responsible, at least in part, for the gender difference described in renal PAH secretion.
已观察到肾皮质刷状缘（BBMV）和基底外侧（BLMV）膜囊泡中对氨基马尿酸（PAH）转运动力学参数的性别差异。各向异性值表明，雌性大鼠BBMV的膜流动性高于雄性大鼠（雌性和雄性分别为0.1897±0.0010和0.2003±0.0014，p<0.05）。两性的BLMV膜流动性相似。蛋白质印迹研究表明，雌性BLMV中OAT1蛋白的含量仅为雄性大鼠BLMV中含量的40%。在雌性中观察到的BLMV中OAT1表达较低，同时BBMV流动性较高（这可能影响该膜结构域中PAH转运体的亲和力），这可能至少部分导致了肾PAH分泌中描述的性别差异。
