Folwaczny Matthias, Heym Richard, Mehl Albert, Hickel Reinhard
Department of Operative Dentistry and Periodontology, Ludwig-Maximilians University, Munich, Germany.
J Periodontol. 2002 Jun;73(6):597-601. doi: 10.1902/jop.2002.73.6.597.
It has been suggested that the 655 nm InGaAsP diode laser radiation can induce considerably higher amounts of fluorescence in subgingival calculus than in cementum. This in vitro study evaluated the use of fluorescence induced by the diode laser radiation for the detection of subgingival calculus.
The study sample consisted of 30 extracted molars, premolars, canines, and incisors. The root surface of each sample was partially covered with subgingival calculus. The source of laser radiation was an InGaAsP diode laser emitting visible radiation at a wavelength of 655 nm. The fluorescent radiation reflected from the cementum and calculus was detected by a photoelectric cell integrated within the diagnostic system. The samples were randomly assigned to 3 experimental groups of 10 teeth each for detection of calculus in various media. Specifically, the fluorescence was determined in air (group A), in electrolytic salt solution (group B), and in blood (group C). For each sample, the intensity of the fluorescent light was evaluated separately for cementum and calculus in triplicate measurements. The statistical analysis was performed using a t test at a level of significance of 5% (P<0.05).
The intensities of fluorescence radiation emitted by cementum were 0.4 (+/-0.51) in air, 0.4 (+/-0.51) in electrolytic solution, and 2.13 (+/-0.59) in blood. For calculus, the relative intensities of fluorescence radiation were 54.1 (+/-29.09) in air, 60.77 (+/-28.35) in electrolytic salt solution, and 39.63 (+/-29.79) in blood. The differences in fluorescence between cementum and calculus within each experimental unit were significant (group A: P= 0.0002, group B: P= 0.0001, group C: P= 0.031). The intensity of fluorescence in calculus was also significantly different between the 3 experimental groups.
The present study indicates that subgingival calculus can be reliably detected on extracted teeth using laser fluorescence induced by the 655 nm diode-laser radiation. In vivo studies are necessary to evaluate the clinical utility of this procedure.
有人提出，655纳米的铟镓砷磷二极管激光辐射在龈下牙石中诱导产生的荧光量比在牙骨质中要高得多。这项体外研究评估了二极管激光辐射诱导的荧光用于检测龈下牙石的情况。
研究样本包括30颗拔除的磨牙、前磨牙、尖牙和切牙。每个样本的牙根表面部分覆盖有龈下牙石。激光辐射源是一台发射波长为655纳米可见光的铟镓砷磷二极管激光。从牙骨质和牙石反射的荧光辐射由诊断系统内的光电管检测。样本被随机分为3个实验组，每组10颗牙齿，用于在不同介质中检测牙石。具体而言，在空气中（A组）、电解盐溶液中（B组）和血液中（C组）测定荧光。对于每个样本，分别对牙骨质和牙石的荧光强度进行三次重复测量评估。使用t检验进行统计分析，显著性水平为5%（P<0.05）。
牙骨质发出的荧光辐射强度在空气中为0.4（±0.51），在电解溶液中为0.4（±0.51），在血液中为2.13（±0.59）。对于牙石，荧光辐射的相对强度在空气中为54.1（±29.09），在电解盐溶液中为60.77（±28.35），在血液中为39.63（±29.79）。每个实验单位中牙骨质和牙石之间的荧光差异显著（A组：P = 0.0002，B组：P = 0.0001，C组：P = 0.031）。3个实验组之间牙石中的荧光强度也有显著差异。
本研究表明，使用655纳米二极管激光辐射诱导的激光荧光可以可靠地在拔除的牙齿上检测龈下牙石。有必要进行体内研究以评估该方法的临床实用性。
