Marshavina Z V, Gazaryan V A
Prikl Biokhim Mikrobiol. 1975 May-Jun;11(3):356-61.
The effect of oxidized and unoxidized oleic acid and Tween-80 on the growth and lysine synthesis by the producers C. glutamicum strains 95, 8, 28 was investigated. Surface active substances like oxidized and unoxidized oleic acid and Tween-80 during cultivation of the lysine producers on the glucose medium (the synthetic medium) and the medium with molasses and corn extract either inhibited the culture growth, thus reducing lysine yield, or accelerated the culture growth, thus increasing lysine yield. Oxidized and unoxidized oleic acid produced the greatest effect when added to the nutrient medium on the 48th cultivation hour. The increment of synthesized lysine was 120-150% of the control. Tween-80 proved to be very effective when added at early stages of fermentation (20 hours).
研究了氧化型和未氧化型油酸以及吐温 - 80对谷氨酸棒杆菌95、8、28菌株生长和赖氨酸合成的影响。在赖氨酸生产菌于葡萄糖培养基（合成培养基）以及含有糖蜜和玉米提取物的培养基上培养过程中，氧化型和未氧化型油酸以及吐温 - 80等表面活性物质，要么抑制培养物生长，从而降低赖氨酸产量，要么加速培养物生长，从而提高赖氨酸产量。氧化型和未氧化型油酸在培养的第48小时添加到营养培养基中时产生的影响最大。合成赖氨酸的增量为对照的120 - 150%。事实证明，吐温 - 80在发酵早期（20小时）添加时非常有效。
