吐温80对血清叶酸酪酸梭状芽孢杆菌检测潜伏期的影响。
Effect of Tween 80 on incubation period of Lactobacillus casei assay of serum folate.
作者信息
Tennant G B
出版信息
J Clin Pathol. 1978 Mar;31(3):227-9. doi: 10.1136/jcp.31.3.227.
Abstract
Contamination of folic acid assay medium with oleate led to a lag phase when Lactobacillus casei cultures were grown on folic acid but not when whole serum provided the folate source. This led to high assay values when using 20-hour incubation periods but satisfactory results were obtained after 48 hours' incubation. The addition of Tween 80 to the medium eliminated the lag phase and the assay results were consistent after 13, 24, and 48 hours' incubation.
摘要
当在叶酸上培养干酪乳杆菌培养物时，油酸对叶酸测定培养基的污染导致了一个滞后期，但当全血清作为叶酸来源时则不会出现这种情况。这导致在使用20小时孵育期时测定值较高，但在48小时孵育后获得了满意的结果。向培养基中添加吐温80消除了滞后期，并且在13、24和48小时孵育后测定结果一致。
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Effect of Tween 80 on incubation period of Lactobacillus casei assay of serum folate.吐温80对血清叶酸酪酸梭状芽孢杆菌检测潜伏期的影响。
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