吐温80对血清叶酸酪酸梭状芽孢杆菌检测潜伏期的影响。
Effect of Tween 80 on incubation period of Lactobacillus casei assay of serum folate.
作者信息
Tennant G B
出版信息
J Clin Pathol. 1978 Mar;31(3):227-9. doi: 10.1136/jcp.31.3.227.
Abstract
Contamination of folic acid assay medium with oleate led to a lag phase when Lactobacillus casei cultures were grown on folic acid but not when whole serum provided the folate source. This led to high assay values when using 20-hour incubation periods but satisfactory results were obtained after 48 hours' incubation. The addition of Tween 80 to the medium eliminated the lag phase and the assay results were consistent after 13, 24, and 48 hours' incubation.
摘要
当在叶酸上培养干酪乳杆菌培养物时，油酸对叶酸测定培养基的污染导致了一个滞后期，但当全血清作为叶酸来源时则不会出现这种情况。这导致在使用20小时孵育期时测定值较高，但在48小时孵育后获得了满意的结果。向培养基中添加吐温80消除了滞后期，并且在13、24和48小时孵育后测定结果一致。
相似文献
1
Effect of Tween 80 on incubation period of Lactobacillus casei assay of serum folate.
J Clin Pathol. 1978 Mar;31(3):227-9. doi: 10.1136/jcp.31.3.227.
2
Continuous-flow automation of the Lactobacillus casei serum folate assay.
J Clin Pathol. 1977 Dec;30(12):1168-74. doi: 10.1136/jcp.30.12.1168.
3
New Tween-80 microbiological assay of serum folate levels in humans and animals.
J AOAC Int. 2012 Sep-Oct;95(5):1505-10. doi: 10.5740/jaoacint.11-071.
4
Cryoprotected Lactobacillus casei: an approach to standardization of microbiological assay of folic acid in serum.
Clin Chem. 1981 May;27(5):745-7.
5
Studies on the response of Lactobacillus casei to different folate monoglutamates.
Br J Nutr. 1982 Mar;47(2):183-9. doi: 10.1079/bjn19820026.
6
Folate transport in Lactobacillus casei: solubilization and general properties of the binding protein.
Biochem Biophys Res Commun. 1976 Feb 9;68(3):712-7. doi: 10.1016/0006-291x(76)91203-1.
7
Effects of antineoplastic drugs on Lactobacillus casei and radioisotopic assays for serum folate.
Am J Clin Pathol. 1978 Feb;69(2):137-9. doi: 10.1093/ajcp/69.2.137.
8
The threshold growth response of Lactobacillus casei to 5-methyl-tetrahydrofolic acid: implications for folate assays.
Br J Nutr. 1985 May;53(3):569-73. doi: 10.1079/bjn19850066.
9
Experience with microbiological assay for folate using a chloramphenicol-resistant L. casei strain.
J Clin Pathol. 1972 Dec;25(12):1050-2. doi: 10.1136/jcp.25.12.1050.
10
Effect of incubation time on Lactobacillus casei bioassay. Variation with two different commercial assay media.
Am J Clin Pathol. 1974 Dec;62(6):840-5. doi: 10.1093/ajcp/62.6.840.
本文引用的文献
1
Inhibition of a strain of Brucella abortus by medium filtered through cotton.
Public Health Rep (1896). 1951 Jan 12;66(2):44-9.
2
Role of fatty acids in the growth stimulation of Sarcina species by vitamin-free casein digests.
J Bacteriol. 1959 Dec;78(6):839-43. doi: 10.1128/jb.78.6.839-843.1959.
3
Folic acid, its analogs and antagonists.
Adv Clin Chem. 1960;3:235-97. doi: 10.1016/s0065-2423(08)60101-8.
4
Effect of incubation time on the L. casei bioassay of folic acid in serum.
Blood. 1969 Aug;34(2):216-21.
5
Stimulation by fatty acids of amino acid accumulation in pantothenic acid depleted Lactobacillus plantarum.
Biochem Biophys Res Commun. 1972 Jan 31;46(2):437-42. doi: 10.1016/s0006-291x(72)80157-8.
6
Effect of incubation time on Lactobacillus casei bioassay. Variation with two different commercial assay media.
Am J Clin Pathol. 1974 Dec;62(6):840-5. doi: 10.1093/ajcp/62.6.840.
7
A comparison of seven strains of Lactobacillus casei var. rhamnosus in relation to their use in the microbiological assay of serum folate.
J Appl Bacteriol. 1974 Sep;37(3):373-89. doi: 10.1111/j.1365-2672.1974.tb00453.x.
8
Effect of incubation time on folate values.
Am J Clin Pathol. 1974 Jan;61(1):74-7. doi: 10.1093/ajcp/61.1.74.
Zotero 插件