Antoniou Dimitri, Caratzoulas Stavros, Kalyanaraman C, Mincer Joshua S, Schwartz Steven D
Department of Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
Eur J Biochem. 2002 Jul;269(13):3103-12. doi: 10.1046/j.1432-1033.2002.03021.x.
This review describes studies of particular enzymatically catalyzed reactions to investigate the possibility that catalysis is mediated by protein dynamics. That is, evolution has crafted the protein backbone of the enzyme to direct vibrations in such a fashion to speed reaction. The review presents the theoretical approach we have used to investigate this problem, but it is designed for the nonspecialist. The results show that in alcohol dehydrogenase, dynamic protein motion is in fact strongly coupled to chemical reaction in such a way as to promote catalysis. This result is in concert with both experimental data and interpretations for this and other enzyme systems studied in the laboratories of the two other investigators who have published reviews in this issue.
本综述描述了对特定酶催化反应的研究，以探讨催化作用是否由蛋白质动力学介导。也就是说，进化塑造了酶的蛋白质骨架，使其以特定方式引导振动从而加速反应。该综述介绍了我们用于研究此问题的理论方法，其面向非专业人士。结果表明，在乙醇脱氢酶中，动态蛋白质运动实际上与化学反应紧密耦合，从而促进催化作用。这一结果与另外两位在本期发表综述的研究者实验室中所研究的该酶系统及其他酶系统的实验数据和解释相一致。
