Silveira P F, Breno M C, Martín del Río M P, Mancera J M
Departamento de Biología Animal, Facultad de Ciencias del Mar, Universidad de Cádiz, 11510 Puerto Real Cádiz, Spain.
J Chem Neuroanat. 2002 Jun;24(1):15-26. doi: 10.1016/s0891-0618(02)00016-9.
Polyclonal antibodies against vasotocin (AVT) and mesotocin (MST) were used to explore the distribution of these peptides in the brain of the snake Bothrops jararaca. Magnocellular AVT- and MST-immunoreactive (ir) perikarya were observed in the supraoptic nucleus (SON), being AVT-ir neurons more numerous. A portion of the SON, in the lateroventral margin of the diencephalon ventrally to optic tract, showed only AVT-ir perikarya and fibers. However, the caudal most portion displayed only mesotocinergic perikarya. Parvocellular and magnocellular AVT- and MST-ir perikarya were present in the paraventricular nucleus (PVN) being AVT-ir fibers more abundant than MST-ir. Vasotocinergic perikarya were also found in a dorsolateral aggregation (DLA) far from the PVN. Mesotocinergic perikarya were also present in the recessus infundibular nucleus and ependyma near to paraventricular organ. Nerve fibers emerging from supraoptic and paraventricular nuclei run along the diencephalic floor, internal zone of the median eminence (ME) to end in the neural lobe. Also a dense network of AVT- and MST-ir fibers was present in the external zone of the ME, close to the vessels of the hypophysial portal system. As a rule, all regions having vasotocinergic and mesotocinergic perikarya also showed immunoreactive fibers. Vasotocinergic and mesotocinergic fibers but not perikarya were found in the lamina terminalis (LT). Moreover AVT-ir fibers were present in the nucleus accumbens and MST-ir fibers in the septum. In mesencephalon and rhombencephalon MST-ir fibers were more numerous than AVT-ir fibers. Vasotocinergic and mesotocinergic fibers in extrahypothalamic areas suggest that these peptides could function as neurotransmitters and/or neuromodulators in the snake B. jararaca.
使用抗血管紧张素（AVT）和中肾素（MST）的多克隆抗体来探究这些肽在巴西矛头蝮蛇脑中的分布。在视上核（SON）中观察到了大细胞AVT和MST免疫反应性（ir）神经元胞体，其中AVT-ir神经元数量更多。在间脑腹侧视束下方的外侧腹缘区域，SON的一部分仅显示AVT-ir神经元胞体和纤维。然而，最尾端部分仅显示中肾素能神经元胞体。室旁核（PVN）中存在小细胞和大细胞AVT和MST-ir神经元胞体，其中AVT-ir纤维比MST-ir纤维更丰富。在远离PVN的背外侧聚集区（DLA）也发现了血管紧张素能神经元胞体。中肾素能神经元胞体也存在于漏斗隐窝核和室旁器官附近的室管膜中。从视上核和室旁核发出的神经纤维沿着间脑底部、正中隆起（ME）的内侧区延伸至神经叶末端。此外，在ME的外侧区靠近垂体门脉系统血管处也存在密集的AVT和MST-ir纤维网络。通常，所有具有血管紧张素能和中肾素能神经元胞体的区域也显示有免疫反应性纤维。在终板（LT）中发现了血管紧张素能和中肾素能纤维，但没有神经元胞体。此外，伏隔核中有AVT-ir纤维，隔区中有MST-ir纤维。在中脑和后脑，MST-ir纤维比AVT-ir纤维更多。下丘脑外区域的血管紧张素能和中肾素能纤维表明，这些肽在巴西矛头蝮蛇中可能作为神经递质和/或神经调节剂发挥作用。
