Mancera J M, López Avalos M D, Pérez-Fígares J M, Fernández-Llebrez P
Departamento de Biología Celular, Facultad de Ciencias, Málaga, España.
Cell Tissue Res. 1991 Jun;264(3):539-48. doi: 10.1007/BF00319043.
The anatomical distribution of neurons and nerve fibers containing corticotropin-releasing factor (CRF) has been studied in the brain of the snake, Natrix maura, by means of immunocytochemistry using an antiserum against rat CRF. To test the possible coexistence of CRF with the neurohypophysial peptides arginine vasotocin (AVT) and mesotocin (MST) adjacent sections were stained with antisera against the two latter peptides. CRF-immunoreactive (CRF-IR) neurons exist in the paraventricular nucleus (PVN). In some neurons of the PVN, coexistence of CRF with MST or of CRF with AVT has been shown. Numerous CRF-IR fibers run along the hypothalamo-hypophysial tract and end in the outer layer of the median eminence. In addition, some fibers reach the neural lobe of the hypophysis. CRF-IR perikarya have also been identified in the following locations: dorsal cortex, nucleus accumbens, amygdala, subfornical organ, lamina terminalis, nucleus of the paraventricular organ, nucleus of the oculomotor nerve, nucleus of the trigeminal nerve, and reticular formation. In addition to all these locations CRF-IR fibers were also observed in the lateral septum, supraoptic nucleus, habenula, lateral forebrain bundle, paraventricular organ, hypothalamic ventromedial nucleus, raphe and interpeduncular nuclei.
利用抗大鼠促肾上腺皮质激素释放因子（CRF）的抗血清，通过免疫细胞化学方法，对麻蛇（Natrix maura）脑内含有CRF的神经元和神经纤维的解剖分布进行了研究。为了检测CRF与神经垂体肽精氨酸血管加压素（AVT）和中催产素（MST）可能的共存情况，相邻切片用抗后两种肽的抗血清进行染色。CRF免疫反应性（CRF-IR）神经元存在于室旁核（PVN）。在PVN的一些神经元中，已显示CRF与MST或CRF与AVT共存。许多CRF-IR纤维沿下丘脑-垂体束走行，并终止于正中隆起的外层。此外，一些纤维到达垂体神经叶。在以下部位也发现了CRF-IR核周体：背侧皮质、伏隔核、杏仁核、穹窿下器官、终板、室旁器官核、动眼神经核、三叉神经核和网状结构。除了所有这些部位外，在外侧隔、视上核、缰核、外侧前脑束、室旁器官、下丘脑腹内侧核、中缝核和脚间核中也观察到了CRF-IR纤维。
